Iron deficiency cause changes in photochemistry, thylakoid organization, and accumulation of photosystem II proteins in Chlamydomonas reinhardtii

Elsin Raju Devadasu, Sai Kiran Madireddi, Srilatha Nama, S. Rajagopal

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A trace element, iron (Fe) plays a pivotal role in photosynthesis process which in turn mediates the plant growth and productivity. Here, we have focused majorly on the photochemistry of photosystem (PS) II, abundance of proteins, and organization of supercomplexes of thylakoids from Fe-depleted cells in Chlamydomonas reinhardtii. Confocal pictures show that the cell's size has been reduced and formed rosette-shaped palmelloids; however, there is no cell death. Further, the PSII photochemistry was reduced remarkably. Further, the photosynthetic efficiency analyzer data revealed that both donor and acceptor side of PSII were equally damaged. Additionally, the room-temperature emission spectra showed the fluorescence emission maxima increased due to impaired energy transfer from PSII to PSI. Furthermore, the protein data reveal that most of the proteins of reaction center and light-harvesting antenna were reduced in Fe-depleted cells. Additionally, the supercomplexes of PSI and PSII were destabilized from thylakoids under Fe-deficient condition showing that Fe is an important element in photosynthesis mechanism.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalPhotosynthesis Research
DOIs
Publication statusAccepted/In press - Jun 21 2016

Fingerprint

Photochemistry
Chlamydomonas reinhardtii
Thylakoids
Photosystem II Protein Complex
photochemistry
Photochemical reactions
thylakoids
photosystem II
Iron
Photosynthesis
iron
photosynthesis
Proteins
light harvesting complex
proteins
Energy Transfer
Trace Elements
Cell death
cells
energy transfer

Keywords

  • Blue native gel electrophoresis
  • Fe deficiency
  • Light-harvesting complexes
  • Photochemistry
  • Photosystem II
  • Thylakoid organization

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry

Cite this

Iron deficiency cause changes in photochemistry, thylakoid organization, and accumulation of photosystem II proteins in Chlamydomonas reinhardtii. / Devadasu, Elsin Raju; Madireddi, Sai Kiran; Nama, Srilatha; Rajagopal, S.

In: Photosynthesis Research, 21.06.2016, p. 1-10.

Research output: Contribution to journalArticle

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