IP3 type 1 receptors in the heart: Their predominance in atrial walls with ganglion cells

J. Slavikova, M. Dvorakova, J. Reischig, M. Palkovits, K. Ondrias, B. Tarabova, L. Lacinova, R. Kvetnansky, A. Marks, O. Krizanova

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Previously we have shown that inositol 1,4,5-trisphosphate (IP3) receptors (IP3Rs) are abundantly expressed in the atria of rat hearts. Since arrangement of atria is very heterogeneous, in this work we focused on the precise localization of IP3 receptors in the left atrium, where the gene expression of the type 1 IP3R was the highest. The mRNA levels of the IP3 type 1 receptors in the left atrium, left ventricle and myocytes were determined using real-time polymerase chain reaction and Taqman probe. For precise localization, immunohistochemistry with the antibody against type 1 IP3Rs was performed. The mRNA of type 1 IP3 receptor was more than three times higher in the left atrium than in the left ventricle, as determined by real-time PCR. Expression of the type 1 IP3 receptor mRNA was higher in the atria, especially in parts containing cardiac ganglion cells. The atrial auricles, which are particularly free of ganglion cells, and the ventricles (wall of the right and left ventricle and ventricular septum) contained four to five times less IP3 receptors than atrial samples with ganglia. IP3R type 1 immunoreactivity detected by a confocal microscope attributed the most condensed signal on ganglionic cells, although light immunoreactivity was also seen in cardiomyocytes. These results show that type 1IP3 receptors predominate in intrinsic neuronal ganglia of cardiac atria.

Original languageEnglish
Pages (from-to)1598-1602
Number of pages5
JournalLife Sciences
Volume78
Issue number14
DOIs
Publication statusPublished - Feb 28 2006

Keywords

  • Cardiac atria
  • Cardiac ganglia
  • IP receptors
  • Myocytes
  • Real-time PCR

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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    Slavikova, J., Dvorakova, M., Reischig, J., Palkovits, M., Ondrias, K., Tarabova, B., Lacinova, L., Kvetnansky, R., Marks, A., & Krizanova, O. (2006). IP3 type 1 receptors in the heart: Their predominance in atrial walls with ganglion cells. Life Sciences, 78(14), 1598-1602. https://doi.org/10.1016/j.lfs.2005.07.012