Ionic events during the volume response of human peripheral blood lymphocytes to hypotonic media: I. Distinctions Between Volume-activated Cl and K+ Conductance Pathways

B. Sarkadi, Esther Mack, A. Rothstein

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Human peripheral blood lymphocytes (PBL), when placed into hypotonic media, first swell and then shrink back to their original volumes because of a rapid KCl leakage via volume-activated K+ and anion permeation pathways. By using gramicidin, a cation channel-forming ionophore, cation transport through the cell membrane can be shunted so that the salt fluxes and thus the volume changes are limited by the rate of the net anion movements. The "gramicidin method," supplemented with direct measurements ofvolumeinduced ion fluxes, can be used to assess the effects of drugs and of various treatments on cation and anion permeabilities. It is demonstrated that quinine and cetiedil are much more effective blockers of volume-induced K+ transport than of Cl transport, while dipyridamole, DIDS, and NIP-taurine inhibit only volume-induced Cl movement. Oligomycins block both cation and anion transport pathways, oligomycin A being more effective in inhibiting K+ transport and oligomycin C preferentially blocking Cl movement. Ca depletion of PBL abolishes volume-induced K+ transport but has no effect on Cl transport. Repletion of cell calcium by ionophore A23187 immediately restores rapid K+ transport without significantly affecting volume-induced Cl transport. These observations, taken together with other reported information, can be best explained by a model in which cell swelling activates independent CI and K+ conductance pathways, the latter being similar in properties to the Ca2+- activated K+ transport observed in various cell membranes.

Original languageEnglish
Pages (from-to)497-512
Number of pages16
JournalJournal of General Physiology
Volume83
Issue number4
DOIs
Publication statusPublished - Apr 1 1984

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Ionic events during the volume response of human peripheral blood lymphocytes to hypotonic media: I. Distinctions Between Volume-activated Cl<sup>−</sup> and K<sup>+</sup> Conductance Pathways'. Together they form a unique fingerprint.

  • Cite this