Ischemia- and reperfusion-induced time dependent changes of the intracellular K+/K(i)+/, Na+/Na(i)+/ and Ca2+/Ca2(i)+/ concentrations were analyzed in isolated Langendorff guinea pig hearts. In the ischemic period evoked by complete perfusion stop of 25 min duration a rapid loss of K+ and gain of Na+ was found. Ca2(i)+ concentration started to increase only after 15 min of global ischemia. Reperfusion did not evoke substantial changes during the first minutes however Ca2(i)+ very rapidly increased after 7 minutes of reperfusion. K(i)+ rapidly increased above initial values in the first 3 minutes of reperfusion but then suddenly declined to the lowest ischemic level and remained at this level. Na(i)+ concentration elevated by ischemia was not further affected by reperfusion. The membrane stabilizing antiarrhythmic agents: quinidine and lidocaine proved to be effective in preventing or moderating ischemia induced shifts in K(i)+ and Na(i)+ concentrations however did not affect Ca2(i)+ content. This latter was markedly reduced by verapamil, a substance which failed to influence ischemic shifts of K(i)+ and Na(i)+ content.
|Journal||Biomedica Biochimica Acta|
|Publication status||Published - Dec 1 1987|
ASJC Scopus subject areas