Ion and water distribution in pig lenses incubated at 0 degree C to disable ion transport pumps.

I. L. Cameron, W. E. Hardman, G. D. Fullerton, M. Kellermayer, A. Ludany, A. Miseta

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study was designed to test how extended exposure of lenses to sera with different ionic strengths influences the distribution of ions and water in the lens. Pig lenses were incubated in cold sera (0 degree C), which were adjusted to variable concentrations of NaCl, and their K+, Na+, Cl-, and water contents were measured. Incubation at 0 degree C inhibits active transport processes and thereby allows equilibration of the mobile ions and water. The hypothesis was that lens water content (volume) would follow the ion-induced protein changes predicted by a model derived from previous osmotic studies on proteins. As expected, exposure of the lens to cold caused a gain of sodium and a partial loss of potassium. However, the potassium concentration in the lens remained several fold higher than that in the bathing solution (about 41 vs. 1.8-4.6 mM/kg H2O), indicating that a portion of the potassium within the cold-exposed lens was not free to diffuse. That the water content of the lens showed a negative rather than a positive relationship with the concentration of NaCl within the lens was explained by the idea that an increase in NaCl within the lens (up to at least 250 mM/kg H2O) causes a decrease in the osmotically unresponsive water volume associated with lens proteins.

Original languageEnglish
Pages (from-to)742-746
Number of pages5
JournalBiochemistry and Cell Biology
Volume69
Issue number10-11
Publication statusPublished - Oct 1991

Fingerprint

Ion Pumps
Ion Transport
Lenses
Swine
Pumps
Ions
Water
Water content
Potassium
Crystallins
Active Biological Transport
Ionic strength
Serum
Osmolar Concentration
Proteins
Sodium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Cameron, I. L., Hardman, W. E., Fullerton, G. D., Kellermayer, M., Ludany, A., & Miseta, A. (1991). Ion and water distribution in pig lenses incubated at 0 degree C to disable ion transport pumps. Biochemistry and Cell Biology, 69(10-11), 742-746.

Ion and water distribution in pig lenses incubated at 0 degree C to disable ion transport pumps. / Cameron, I. L.; Hardman, W. E.; Fullerton, G. D.; Kellermayer, M.; Ludany, A.; Miseta, A.

In: Biochemistry and Cell Biology, Vol. 69, No. 10-11, 10.1991, p. 742-746.

Research output: Contribution to journalArticle

Cameron, IL, Hardman, WE, Fullerton, GD, Kellermayer, M, Ludany, A & Miseta, A 1991, 'Ion and water distribution in pig lenses incubated at 0 degree C to disable ion transport pumps.', Biochemistry and Cell Biology, vol. 69, no. 10-11, pp. 742-746.
Cameron IL, Hardman WE, Fullerton GD, Kellermayer M, Ludany A, Miseta A. Ion and water distribution in pig lenses incubated at 0 degree C to disable ion transport pumps. Biochemistry and Cell Biology. 1991 Oct;69(10-11):742-746.
Cameron, I. L. ; Hardman, W. E. ; Fullerton, G. D. ; Kellermayer, M. ; Ludany, A. ; Miseta, A. / Ion and water distribution in pig lenses incubated at 0 degree C to disable ion transport pumps. In: Biochemistry and Cell Biology. 1991 ; Vol. 69, No. 10-11. pp. 742-746.
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