Iodination of peptide hormones and purification of iodinated peptides by HPLC

T. Janáky, G. Tóth, B. Penke, K. Kovács, F. A. László

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Hexagastrin, Arg 8-vasopressin, oxytocin, Tyr1-somatostatin, 1 and ACTH 1–39 were iodinated in order to yield precursors for tritium labelling or radioiodinated tracers for radioimmunoassay, respectively. The heterogeneous mixture of iodination products was purified via reversed-phase high-performance liquid chromatography. Iodination of peptides resulted in a marked increase in retention time on the reversed-phase adsorbent. A simple and quick method was applied for purification of radio-iodinated peptides on a Sep-pak R C-18 cartridge for rapid sample preparation.

Original languageEnglish
Pages (from-to)1499-1507
Number of pages9
JournalJournal of Liquid Chromatography
Issue number8
Publication statusPublished - Jan 1 1982


ASJC Scopus subject areas

  • Molecular Medicine

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