Involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution

K. Kitajka, Cs Buda, E. Fodor, J. E. Halver, T. Farkas

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fluorescence anisotropy parameter of p-(6-phenyl)-1,3,5- hexatrienlyl]phenyl-propionic acid (DPH-PA) and 1-(4- trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) embedded in synaptic plasma membranes prepared from brains of cold (5°C) and warm (22°C) adapted fish (Cyprinus carpio L.), rat (Rattus norvegicus) and bird (Branta canadensis), was studied. Fatty acid composition of total lipids as well as molecular species composition of diacyl phosphatidylcholines and phosphatidylethanolamines was also determined. The amount of long-chain polyunsaturated fatty acids decreased with increasing body temperature. The was a near-complete compensation of membrane structural order for environmental/body temperature over the evolutionary scale as seen by DPH- PA. Using TMA-DPH, the compensation was partial with rat and bird. Since DPH- PA and TMA-DPH differ in their charges, it is proposed, that the former reported membrane regions rich in cationic or zwitterionic (neutral) phospholipids and the latter, membrane regions rich in negatively charged phospholipids in the synaptic plasma membranes. Many different molecular species (20-25) of diacyl phophatidylcholines and diacyl phosphatidylethanolamines were identified. The level of 16:0/22:6 phosphatidylcholine decreased while disaturated phosphatidylcholines increased with increase of environmental/body temperature from the fish through the bird. Level of 1-monoenoic, 2-polyenoic phophatidylethanolamines also decreased with an increase in environmental/body temperature. Experiments using vesicles made of mixed synthetic phosphatidylcholine vesicles made of mixed synthetic phosphatidylcholine vesicles (16:0/16:0, 16:0/18:1, 16:0/22:6 in various proportions) showed that increase in disaturated phosphatidylcholine species does not explain the observed complete adjustment of membrane structural order in synaptic plasma membranes. Change in level of 1-monoenoic, 2-polyenoic phosphatidylethanolamines might be one of the factors involved in controlling the biophysical properties of the membrane according to the temperature.

Original languageEnglish
Pages (from-to)1045-1050
Number of pages6
JournalLipids
Volume31
Issue number10
DOIs
Publication statusPublished - Oct 1996

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution'. Together they form a unique fingerprint.

  • Cite this