Die involvierung des nitric oxids im kardioprotektiven effekt des ischämischen preconditioning im ischämie/reperfusion-modell bei schweinen

Translated title of the contribution: Involvement of nitric oxide in the cardioprotective effect of early ischemic preconditioning in the reperfusion phase in pigs

R. Hemetsberger, A. Pósa, N. Pavo, S. Farhan, C. Csonka, T. Csont, P. Ferdinándy, R. Garamvölgyi, Z. Petrasi, Ö Petnehazy, C. Varga, I. Pavo, F. László, K. Huber, J. Wojta, D. Glogar, Mariann Gyöngyösi

Research output: Contribution to journalArticle

Abstract

Background: We investigated the involvement of myocardial tissue inducible and constitutive NOS (iNOS and cNOS) activities in the cardioprotective effect of early ischaemic preconditioning (IP) in closed-chest pigs subjected to percutaneous balloon coronary occlusion/reperfusion. Methods: Catheter-based 90-min occlusion followed by 60-min reperfusion of the left anterior descending coronary artery (LAD) was performed in 8 pigs (group AMI). IP was applied in 9 pigs (group IP) through 2 cycles of 5-min occlusion and 5-min reperfusion of the LAD before a 90-min occlusion and 60-min reperfusion. Infarct size was expressed as a percentage of the area at risk. Fractional collateral flow reserve FFRcoll was measured with pressure-wire (PW) placed distally to the occluding balloon at IP, and during a 30-sec balloon occlusion of the infarkt related artery after final 60-min reperfusion. Tissue iNOS/cNOS activities were measured in the infarcted area (iNOS-I/cNOS-I) and in the border zone (iNOS-B/cNOS-B) by using of citrullin-assay. Results: Infarct size was smaller in group IP as compared with group AMI (21.7 ± 4.4 vs 27 ± 3.4 %; p = 0.014). IP did not influence the tissue iNOS-I, iNOS-B and cNOS-I activities neither in infarct, or border zone. A trend towards higher border zone cNOS-B activity (49.2 ± 23.2 vs 31 ± 25.1 pmol/min/mg protein; p = 0.142) was measured in group IP vs AMI. PW measurements distally to occlusion site revealed that IP led to higher FFRcoll (0.18 ± 0.06 vs 0.07 ± 0.08; p = 0.031, IP vs AMI), even if it did not reach the value of 0.3 (index for sufficient collateralization). The FFRcoll correlated significantly with cNOS-I (r = 0.726; p = 0.027) and cNOS-B (r = 0.832; p = 0.005) activities in group IP. Discussion: The beneficial effect of cNOS in ischaemia/ reperfusion injury after IP results from the improved myocardial reperfusion after the release of coronary occlusion.

Original languageGerman
Pages (from-to)119-122
Number of pages4
JournalJournal fur Kardiologie
Volume15
Issue number5-6
Publication statusPublished - 2008

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Ischemic Preconditioning
Reperfusion
Nitric Oxide
Swine
Balloon Occlusion
Myocardial Reperfusion
Coronary Occlusion
Pressure
Reperfusion Injury
Coronary Vessels
Thorax
Catheters
Arteries

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Die involvierung des nitric oxids im kardioprotektiven effekt des ischämischen preconditioning im ischämie/reperfusion-modell bei schweinen. / Hemetsberger, R.; Pósa, A.; Pavo, N.; Farhan, S.; Csonka, C.; Csont, T.; Ferdinándy, P.; Garamvölgyi, R.; Petrasi, Z.; Petnehazy, Ö; Varga, C.; Pavo, I.; László, F.; Huber, K.; Wojta, J.; Glogar, D.; Gyöngyösi, Mariann.

In: Journal fur Kardiologie, Vol. 15, No. 5-6, 2008, p. 119-122.

Research output: Contribution to journalArticle

Hemetsberger, R, Pósa, A, Pavo, N, Farhan, S, Csonka, C, Csont, T, Ferdinándy, P, Garamvölgyi, R, Petrasi, Z, Petnehazy, Ö, Varga, C, Pavo, I, László, F, Huber, K, Wojta, J, Glogar, D & Gyöngyösi, M 2008, 'Die involvierung des nitric oxids im kardioprotektiven effekt des ischämischen preconditioning im ischämie/reperfusion-modell bei schweinen', Journal fur Kardiologie, vol. 15, no. 5-6, pp. 119-122.
Hemetsberger, R. ; Pósa, A. ; Pavo, N. ; Farhan, S. ; Csonka, C. ; Csont, T. ; Ferdinándy, P. ; Garamvölgyi, R. ; Petrasi, Z. ; Petnehazy, Ö ; Varga, C. ; Pavo, I. ; László, F. ; Huber, K. ; Wojta, J. ; Glogar, D. ; Gyöngyösi, Mariann. / Die involvierung des nitric oxids im kardioprotektiven effekt des ischämischen preconditioning im ischämie/reperfusion-modell bei schweinen. In: Journal fur Kardiologie. 2008 ; Vol. 15, No. 5-6. pp. 119-122.
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abstract = "Background: We investigated the involvement of myocardial tissue inducible and constitutive NOS (iNOS and cNOS) activities in the cardioprotective effect of early ischaemic preconditioning (IP) in closed-chest pigs subjected to percutaneous balloon coronary occlusion/reperfusion. Methods: Catheter-based 90-min occlusion followed by 60-min reperfusion of the left anterior descending coronary artery (LAD) was performed in 8 pigs (group AMI). IP was applied in 9 pigs (group IP) through 2 cycles of 5-min occlusion and 5-min reperfusion of the LAD before a 90-min occlusion and 60-min reperfusion. Infarct size was expressed as a percentage of the area at risk. Fractional collateral flow reserve FFRcoll was measured with pressure-wire (PW) placed distally to the occluding balloon at IP, and during a 30-sec balloon occlusion of the infarkt related artery after final 60-min reperfusion. Tissue iNOS/cNOS activities were measured in the infarcted area (iNOS-I/cNOS-I) and in the border zone (iNOS-B/cNOS-B) by using of citrullin-assay. Results: Infarct size was smaller in group IP as compared with group AMI (21.7 ± 4.4 vs 27 ± 3.4 {\%}; p = 0.014). IP did not influence the tissue iNOS-I, iNOS-B and cNOS-I activities neither in infarct, or border zone. A trend towards higher border zone cNOS-B activity (49.2 ± 23.2 vs 31 ± 25.1 pmol/min/mg protein; p = 0.142) was measured in group IP vs AMI. PW measurements distally to occlusion site revealed that IP led to higher FFRcoll (0.18 ± 0.06 vs 0.07 ± 0.08; p = 0.031, IP vs AMI), even if it did not reach the value of 0.3 (index for sufficient collateralization). The FFRcoll correlated significantly with cNOS-I (r = 0.726; p = 0.027) and cNOS-B (r = 0.832; p = 0.005) activities in group IP. Discussion: The beneficial effect of cNOS in ischaemia/ reperfusion injury after IP results from the improved myocardial reperfusion after the release of coronary occlusion.",
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T1 - Die involvierung des nitric oxids im kardioprotektiven effekt des ischämischen preconditioning im ischämie/reperfusion-modell bei schweinen

AU - Hemetsberger, R.

AU - Pósa, A.

AU - Pavo, N.

AU - Farhan, S.

AU - Csonka, C.

AU - Csont, T.

AU - Ferdinándy, P.

AU - Garamvölgyi, R.

AU - Petrasi, Z.

AU - Petnehazy, Ö

AU - Varga, C.

AU - Pavo, I.

AU - László, F.

AU - Huber, K.

AU - Wojta, J.

AU - Glogar, D.

AU - Gyöngyösi, Mariann

PY - 2008

Y1 - 2008

N2 - Background: We investigated the involvement of myocardial tissue inducible and constitutive NOS (iNOS and cNOS) activities in the cardioprotective effect of early ischaemic preconditioning (IP) in closed-chest pigs subjected to percutaneous balloon coronary occlusion/reperfusion. Methods: Catheter-based 90-min occlusion followed by 60-min reperfusion of the left anterior descending coronary artery (LAD) was performed in 8 pigs (group AMI). IP was applied in 9 pigs (group IP) through 2 cycles of 5-min occlusion and 5-min reperfusion of the LAD before a 90-min occlusion and 60-min reperfusion. Infarct size was expressed as a percentage of the area at risk. Fractional collateral flow reserve FFRcoll was measured with pressure-wire (PW) placed distally to the occluding balloon at IP, and during a 30-sec balloon occlusion of the infarkt related artery after final 60-min reperfusion. Tissue iNOS/cNOS activities were measured in the infarcted area (iNOS-I/cNOS-I) and in the border zone (iNOS-B/cNOS-B) by using of citrullin-assay. Results: Infarct size was smaller in group IP as compared with group AMI (21.7 ± 4.4 vs 27 ± 3.4 %; p = 0.014). IP did not influence the tissue iNOS-I, iNOS-B and cNOS-I activities neither in infarct, or border zone. A trend towards higher border zone cNOS-B activity (49.2 ± 23.2 vs 31 ± 25.1 pmol/min/mg protein; p = 0.142) was measured in group IP vs AMI. PW measurements distally to occlusion site revealed that IP led to higher FFRcoll (0.18 ± 0.06 vs 0.07 ± 0.08; p = 0.031, IP vs AMI), even if it did not reach the value of 0.3 (index for sufficient collateralization). The FFRcoll correlated significantly with cNOS-I (r = 0.726; p = 0.027) and cNOS-B (r = 0.832; p = 0.005) activities in group IP. Discussion: The beneficial effect of cNOS in ischaemia/ reperfusion injury after IP results from the improved myocardial reperfusion after the release of coronary occlusion.

AB - Background: We investigated the involvement of myocardial tissue inducible and constitutive NOS (iNOS and cNOS) activities in the cardioprotective effect of early ischaemic preconditioning (IP) in closed-chest pigs subjected to percutaneous balloon coronary occlusion/reperfusion. Methods: Catheter-based 90-min occlusion followed by 60-min reperfusion of the left anterior descending coronary artery (LAD) was performed in 8 pigs (group AMI). IP was applied in 9 pigs (group IP) through 2 cycles of 5-min occlusion and 5-min reperfusion of the LAD before a 90-min occlusion and 60-min reperfusion. Infarct size was expressed as a percentage of the area at risk. Fractional collateral flow reserve FFRcoll was measured with pressure-wire (PW) placed distally to the occluding balloon at IP, and during a 30-sec balloon occlusion of the infarkt related artery after final 60-min reperfusion. Tissue iNOS/cNOS activities were measured in the infarcted area (iNOS-I/cNOS-I) and in the border zone (iNOS-B/cNOS-B) by using of citrullin-assay. Results: Infarct size was smaller in group IP as compared with group AMI (21.7 ± 4.4 vs 27 ± 3.4 %; p = 0.014). IP did not influence the tissue iNOS-I, iNOS-B and cNOS-I activities neither in infarct, or border zone. A trend towards higher border zone cNOS-B activity (49.2 ± 23.2 vs 31 ± 25.1 pmol/min/mg protein; p = 0.142) was measured in group IP vs AMI. PW measurements distally to occlusion site revealed that IP led to higher FFRcoll (0.18 ± 0.06 vs 0.07 ± 0.08; p = 0.031, IP vs AMI), even if it did not reach the value of 0.3 (index for sufficient collateralization). The FFRcoll correlated significantly with cNOS-I (r = 0.726; p = 0.027) and cNOS-B (r = 0.832; p = 0.005) activities in group IP. Discussion: The beneficial effect of cNOS in ischaemia/ reperfusion injury after IP results from the improved myocardial reperfusion after the release of coronary occlusion.

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