Involvement of nitric oxide and auxin in signal transduction of copper-induced morphological responses in Arabidopsis seedlings

Andrea Peto, Nóra Lehotai, Jorge Lozano-Juste, José León, I. Tari, L. Erdei, Zsuzsanna Kolbert

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

•Background and Aims: Plants are able to adapt to the environment dynamically through regulation of their growth and development. Excess copper (Cu 2+), a toxic heavy metal, induces morphological alterations in plant organs; however, the underlying mechanisms are still unclear. With this in mind, the multiple signalling functions of nitric oxide (NO) in plant cells and its possible regulatory role and relationship with auxin were examined during Cu 2+-induced morphological responses. •Methods: Endogenous auxin distribution was determined by microscopic observation of X-Gluc-stained DR5::GUS arabidopsis, and the levels of NO, superoxide and peroxynitrite were detected by fluorescence microscopy. As well as wild-type, NO-overproducer (nox1) and -deficient (nia1nia2 and nia1nia2noa1-2) arabidopsis plants were used. •Key Results: Cu 2+ at a concentration of 50 μm resulted in a large reduction in cotyledon area and hypocotyl and primary root lengths, accompanied by an increase in auxin levels. In cotyledons, a low Cu 2+ concentration promoted NO accumulation, which was arrested by nitric oxide synthase or nitrate reductase inhibitors. The 5-μm Cu 2+-induced NO synthesis was not detectable in nia1nia2 or nia1nia2noa1-2 plants. In roots, Cu 2+ caused a decrease of the NO level which was not associated with superoxide and peroxynitrite formation. Inhibition of auxin transport resulted in an increase in NO levels, while exogenous application of an NO donor reduced DR5::GUS expression. The elongation processes of nox1 were not sensitive to Cu 2+, but NO-deficient plants showed diverse growth responses. •Conclusions: In plant organs, Cu 2+ excess results in severe morphological responses during which the endogenous hormonal balance and signal transduction are affected. Auxin and NO negatively regulate each others level and NO intensifies the metal-induced cotyledon expansion, but mitigates elongation processes under Cu 2+ exposure.

Original languageEnglish
Pages (from-to)449-457
Number of pages9
JournalAnnals of Botany
Volume108
Issue number3
DOIs
Publication statusPublished - Sep 2011

Fingerprint

nitric oxide
signal transduction
auxins
copper
Arabidopsis
seedlings
cotyledons
plant organs
superoxide anion
nitrate reductase
nitric oxide synthase
fluorescence microscopy
hypocotyls
growth and development
heavy metals
metals
synthesis

Keywords

  • Arabidopsis thaliana
  • auxin
  • copper
  • morphological responses
  • nitric oxide

ASJC Scopus subject areas

  • Plant Science

Cite this

Involvement of nitric oxide and auxin in signal transduction of copper-induced morphological responses in Arabidopsis seedlings. / Peto, Andrea; Lehotai, Nóra; Lozano-Juste, Jorge; León, José; Tari, I.; Erdei, L.; Kolbert, Zsuzsanna.

In: Annals of Botany, Vol. 108, No. 3, 09.2011, p. 449-457.

Research output: Contribution to journalArticle

Peto, Andrea ; Lehotai, Nóra ; Lozano-Juste, Jorge ; León, José ; Tari, I. ; Erdei, L. ; Kolbert, Zsuzsanna. / Involvement of nitric oxide and auxin in signal transduction of copper-induced morphological responses in Arabidopsis seedlings. In: Annals of Botany. 2011 ; Vol. 108, No. 3. pp. 449-457.
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T1 - Involvement of nitric oxide and auxin in signal transduction of copper-induced morphological responses in Arabidopsis seedlings

AU - Peto, Andrea

AU - Lehotai, Nóra

AU - Lozano-Juste, Jorge

AU - León, José

AU - Tari, I.

AU - Erdei, L.

AU - Kolbert, Zsuzsanna

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