Involvement of FKHR (FOXO1) transcription factor in human uterus leiomyoma growth

Kálmán A. Kovács, Ferenc Lengyel, Ferenc Wilhelm, Zsuzsanna Vértes, B. Sümegi, Marietta Vértes

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: To study the expression of FOXO1 and pSer256-FOXO1 parallel to Akt and pSer473-Akt in leiomyoma compared with adjacent myometrium from human uteri. Design: Prospective study. Setting: University departments. Patient(s): Thirty-eight cyclic and 20 menopausal women who underwent hysterectomy for benign indications. Intervention(s): None. Main Outcome Measure(s): Western blot analyses were used for evaluation in leiomyoma and adjacent myometrium of Akt and pSer473-Akt, 14-3-3 γ proteins and expression and subcellular distribution of FOXO1 and pSer 256-FOXO1 during the menstrual cycle and at menopause. Result(s): The present study demonstrates the expression of FOXO1 and pSer256-FOXO1 at the tissue level in the human uterus leiomyoma and adjacent myometrium. The level of phosphorylated FOXO1 in leiomyoma was higher than in matched myometrium. The pSer256-FOXO1 in leiomyoma during menstrual phases was located mostly in the nuclear fraction comparison to that of the myometrium. The reason for this difference is presumably the simultaneously detected lower level of 14-3-3 protein. Conclusion(s): Abundant level of the phosphorylated FOXO1, its impaired nucleocytoplasmic shuttling, and the lowered expression of 14-3-3 protein in leiomyoma induces a shift in the cellular machinery toward a prosurvival execution program and thus presents a potential therapeutic target for treatment of leiomyoma.

Original languageEnglish
Pages (from-to)1491-1495
Number of pages5
JournalFertility and Sterility
Volume94
Issue number4
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Leiomyoma
Uterus
Myometrium
Transcription Factors
14-3-3 Proteins
Growth
Menstrual Cycle
Menopause
Hysterectomy
Western Blotting
Outcome Assessment (Health Care)
Prospective Studies
Therapeutics

Keywords

  • 14-3-3 γ
  • FOXO1
  • human uterus
  • leiomyoma
  • myometrium
  • nuclear/cytoplasmic shuttling
  • proteins

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Medicine(all)

Cite this

Involvement of FKHR (FOXO1) transcription factor in human uterus leiomyoma growth. / Kovács, Kálmán A.; Lengyel, Ferenc; Wilhelm, Ferenc; Vértes, Zsuzsanna; Sümegi, B.; Vértes, Marietta.

In: Fertility and Sterility, Vol. 94, No. 4, 09.2010, p. 1491-1495.

Research output: Contribution to journalArticle

Kovács, Kálmán A. ; Lengyel, Ferenc ; Wilhelm, Ferenc ; Vértes, Zsuzsanna ; Sümegi, B. ; Vértes, Marietta. / Involvement of FKHR (FOXO1) transcription factor in human uterus leiomyoma growth. In: Fertility and Sterility. 2010 ; Vol. 94, No. 4. pp. 1491-1495.
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