Involvement of ERK and CREB signaling pathways in the protective effect of PACAP in monosodium glutamate-induced retinal lesion

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Abstract

Pituitary adenylate cyclase-activiting polypeptide (PACAP) has well-documented neuroprotective actions, which have also been shown in retinal degeneration induced by monosodium glutamate (MSG) in neonatal rats. The aim of this article was to investigate the activation of extracellular signal-regulated kinase (ERK1/2) and cyclic adenosine 3′,5′-phosphate (cAMP)-responsive element binding protein (CREB) signaling pathways by Western blot analysis in retinal degeneration induced by MSG. We found that intravitreal administration of PACAP preceding the MSG treatments induced significant increases in the phosphorylation, that is, the activation of ERK1/2 and its downstream target, CREB, 12 h after the treatment compared to the contralateral untreated eye during the first two treatments, with no further elevations 24 h after treatments. These results demonstrate that the degenerative effect of MSG and the protective effect of PACAP involve complex kinase signaling pathways and are related to cAMP/ERK/CREB activation.

Original languageEnglish
Pages (from-to)507-511
Number of pages5
JournalAnnals of the New York Academy of Sciences
Volume1070
DOIs
Publication statusPublished - Jul 2006

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Sodium Glutamate
Adenylyl Cyclases
Carrier Proteins
Retinal Degeneration
Peptides
Chemical activation
Adenosine Monophosphate
Phosphorylation
Mitogen-Activated Protein Kinase 1
Extracellular Signal-Regulated MAP Kinases
Adenosine
Rats
Phosphotransferases
Western Blotting
Phosphates
Protein
Lesion
Pathway
Activation
Degeneration

Keywords

  • Intravitreal
  • Neonatal rat
  • Neuroprotection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "Involvement of ERK and CREB signaling pathways in the protective effect of PACAP in monosodium glutamate-induced retinal lesion",
abstract = "Pituitary adenylate cyclase-activiting polypeptide (PACAP) has well-documented neuroprotective actions, which have also been shown in retinal degeneration induced by monosodium glutamate (MSG) in neonatal rats. The aim of this article was to investigate the activation of extracellular signal-regulated kinase (ERK1/2) and cyclic adenosine 3′,5′-phosphate (cAMP)-responsive element binding protein (CREB) signaling pathways by Western blot analysis in retinal degeneration induced by MSG. We found that intravitreal administration of PACAP preceding the MSG treatments induced significant increases in the phosphorylation, that is, the activation of ERK1/2 and its downstream target, CREB, 12 h after the treatment compared to the contralateral untreated eye during the first two treatments, with no further elevations 24 h after treatments. These results demonstrate that the degenerative effect of MSG and the protective effect of PACAP involve complex kinase signaling pathways and are related to cAMP/ERK/CREB activation.",
keywords = "Intravitreal, Neonatal rat, Neuroprotection",
author = "B. R{\'a}cz and A. Tam{\'a}s and P. Kiss and G. T{\'o}th and B. Gasz and B. Borsiczky and A. Ferencz and F. Gallyas and E. Rőth and D. Reglodi",
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AU - Rácz, B.

AU - Tamás, A.

AU - Kiss, P.

AU - Tóth, G.

AU - Gasz, B.

AU - Borsiczky, B.

AU - Ferencz, A.

AU - Gallyas, F.

AU - Rőth, E.

AU - Reglodi, D.

PY - 2006/7

Y1 - 2006/7

N2 - Pituitary adenylate cyclase-activiting polypeptide (PACAP) has well-documented neuroprotective actions, which have also been shown in retinal degeneration induced by monosodium glutamate (MSG) in neonatal rats. The aim of this article was to investigate the activation of extracellular signal-regulated kinase (ERK1/2) and cyclic adenosine 3′,5′-phosphate (cAMP)-responsive element binding protein (CREB) signaling pathways by Western blot analysis in retinal degeneration induced by MSG. We found that intravitreal administration of PACAP preceding the MSG treatments induced significant increases in the phosphorylation, that is, the activation of ERK1/2 and its downstream target, CREB, 12 h after the treatment compared to the contralateral untreated eye during the first two treatments, with no further elevations 24 h after treatments. These results demonstrate that the degenerative effect of MSG and the protective effect of PACAP involve complex kinase signaling pathways and are related to cAMP/ERK/CREB activation.

AB - Pituitary adenylate cyclase-activiting polypeptide (PACAP) has well-documented neuroprotective actions, which have also been shown in retinal degeneration induced by monosodium glutamate (MSG) in neonatal rats. The aim of this article was to investigate the activation of extracellular signal-regulated kinase (ERK1/2) and cyclic adenosine 3′,5′-phosphate (cAMP)-responsive element binding protein (CREB) signaling pathways by Western blot analysis in retinal degeneration induced by MSG. We found that intravitreal administration of PACAP preceding the MSG treatments induced significant increases in the phosphorylation, that is, the activation of ERK1/2 and its downstream target, CREB, 12 h after the treatment compared to the contralateral untreated eye during the first two treatments, with no further elevations 24 h after treatments. These results demonstrate that the degenerative effect of MSG and the protective effect of PACAP involve complex kinase signaling pathways and are related to cAMP/ERK/CREB activation.

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