Involvement of adenosine A2A receptors in engulfment-dependent apoptotic cell suppression of inflammation

Krisztina Köröskényi, Edina Duró, Anna Pallai, Z. Sarang, Doris Kloor, David S. Ucker, Susana Beceiro, Antonio Castrillo, Ajay Chawla, Catherine A. Ledent, L. Fésüs, Z. Szondy

Research output: Contribution to journalArticle

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Abstract

Efficient execution of apoptotic cell death followed by efficient clearance mediated by professional macrophages is a key mechanism in maintaining tissue homeostasis. Removal of apoptotic cells usually involves three central elements: 1) attraction of phagocytes via soluble "find me" signals, 2) recognition and phagocytosis via cell surface-presenting "eat me" signals, and 3) suppression or initiation of inflammatory responses depending on additional innate immune stimuli. Suppression of inflammation involves both direct inhibition of proinflammatory cytokine production and release of anti-inflammatory factors, which all contribute to the resolution of inflammation. In the current study, using wild-type and adenosine A2A receptor (A2AR) null mice, we investigated whether A2ARs, known to mediate anti-inflammatory signals in macrophages, participate in the apoptotic cell-mediated immunosuppression. We found that macrophages engulfing apoptotic cells release adenosine in sufficient amount to trigger A2ARs, and simultaneously increase the expression of A2ARs, as a result of possible activation of liver X receptor and peroxisome proliferators activated receptor d. In macrophages engulfing apoptotic cells, stimulation of A2ARs suppresses the NO-dependent formation of neutrophil migration factors, such as macrophage inflammatory protein-2, using the adenylate cyclase/protein kinase A pathway. As a result, loss of A2ARs results in elevated chemoattractant secretion. This was evident as pronounced neutrophil migration upon exposure of macrophages to apoptotic cells in an in vivo peritonitis model. Altogether, our data indicate that adenosine is one of the soluble mediators released by macrophages that mediate engulfment-dependent apoptotic cell suppression of inflammation.

Original languageEnglish
Pages (from-to)7144-7155
Number of pages12
JournalJournal of Immunology
Volume186
Issue number12
DOIs
Publication statusPublished - Jun 15 2011

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Adenosine A2A Receptors
Macrophages
Inflammation
Adenosine
Neutrophils
Anti-Inflammatory Agents
Chemokine CXCL2
Cytophagocytosis
Peroxisome Proliferator-Activated Receptors
Chemotactic Factors
Phagocytes
Cyclic AMP-Dependent Protein Kinases
Peritonitis
Adenylyl Cyclases
Immunosuppression
Homeostasis
Cell Death
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Involvement of adenosine A2A receptors in engulfment-dependent apoptotic cell suppression of inflammation. / Köröskényi, Krisztina; Duró, Edina; Pallai, Anna; Sarang, Z.; Kloor, Doris; Ucker, David S.; Beceiro, Susana; Castrillo, Antonio; Chawla, Ajay; Ledent, Catherine A.; Fésüs, L.; Szondy, Z.

In: Journal of Immunology, Vol. 186, No. 12, 15.06.2011, p. 7144-7155.

Research output: Contribution to journalArticle

Köröskényi, K, Duró, E, Pallai, A, Sarang, Z, Kloor, D, Ucker, DS, Beceiro, S, Castrillo, A, Chawla, A, Ledent, CA, Fésüs, L & Szondy, Z 2011, 'Involvement of adenosine A2A receptors in engulfment-dependent apoptotic cell suppression of inflammation', Journal of Immunology, vol. 186, no. 12, pp. 7144-7155. https://doi.org/10.4049/jimmunol.1002284
Köröskényi, Krisztina ; Duró, Edina ; Pallai, Anna ; Sarang, Z. ; Kloor, Doris ; Ucker, David S. ; Beceiro, Susana ; Castrillo, Antonio ; Chawla, Ajay ; Ledent, Catherine A. ; Fésüs, L. ; Szondy, Z. / Involvement of adenosine A2A receptors in engulfment-dependent apoptotic cell suppression of inflammation. In: Journal of Immunology. 2011 ; Vol. 186, No. 12. pp. 7144-7155.
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