[INVITED] Novel optical biosensing technologies for detection of mycotoxins

A. Nabok, A. G. Al-Rubaye, A. M. Al-Jawdah, A. Tsargorodska, J. L. Marty, G. Catanante, A. Székács, E. Takács

Research output: Contribution to journalArticle

Abstract

This work reviews our recent progress in development of novel optical methods of detection of mycotoxins in direct assay with either specific antibodies or aptamers. The main method in this work was the total internal reflection ellipsometry (TIRE) combined with LSPR transducers based on gold nano-structures produced by annealing of thin gold films. The gold nano-islands produced were characterised with SEM, AFM, UV–visible absorption spectroscopy, and spectroscopic ellipsometry. The combination of TIRE and LSPR offers superior refractive index sensitivity as compared to traditional UV–vis absorption spectroscopy. The limitations of LSPR related to a short evanescent field decay length can be overcome using small-size bio-receptors, such as half-antibodies and aptamers. The achieved sensitivity of detection of mycotoxins in 0.01 ppb level of concentration is sufficient for the use of this method for analysis of agriculture products, food and feed on the presence of mycotoxins. Even higher sensitivity in sub-ppt level was achieved with another optical biosensor developed recently; it is based on optical planar waveguide operating as polarization interferometer (PI). This method is promising for development of portable, highly sensitive, and simple to use biosensors suitable for point-of-need detection of mycotoxins.

LanguageEnglish
Pages212-221
Number of pages10
JournalOptics and Laser Technology
Volume109
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Mycotoxins
ellipsometry
Gold
Ellipsometry
gold
antibodies
Absorption spectroscopy
bioinstrumentation
Biosensors
Antibodies
absorption spectroscopy
Evanescent fields
Planar waveguides
agriculture
Spectroscopic ellipsometry
sensitivity
Optical waveguides
Ultraviolet spectroscopy
food
Interferometers

Keywords

  • Direct immuno- or aptamer assay
  • LSPR
  • Mycotoxins
  • Optical biosensors
  • Polarization interferometry
  • TIRE

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

Cite this

Nabok, A., Al-Rubaye, A. G., Al-Jawdah, A. M., Tsargorodska, A., Marty, J. L., Catanante, G., ... Takács, E. (2019). [INVITED] Novel optical biosensing technologies for detection of mycotoxins. Optics and Laser Technology, 109, 212-221. https://doi.org/10.1016/j.optlastec.2018.07.076

[INVITED] Novel optical biosensing technologies for detection of mycotoxins. / Nabok, A.; Al-Rubaye, A. G.; Al-Jawdah, A. M.; Tsargorodska, A.; Marty, J. L.; Catanante, G.; Székács, A.; Takács, E.

In: Optics and Laser Technology, Vol. 109, 01.01.2019, p. 212-221.

Research output: Contribution to journalArticle

Nabok A, Al-Rubaye AG, Al-Jawdah AM, Tsargorodska A, Marty JL, Catanante G et al. [INVITED] Novel optical biosensing technologies for detection of mycotoxins. Optics and Laser Technology. 2019 Jan 1;109:212-221. https://doi.org/10.1016/j.optlastec.2018.07.076
Nabok, A. ; Al-Rubaye, A. G. ; Al-Jawdah, A. M. ; Tsargorodska, A. ; Marty, J. L. ; Catanante, G. ; Székács, A. ; Takács, E. / [INVITED] Novel optical biosensing technologies for detection of mycotoxins. In: Optics and Laser Technology. 2019 ; Vol. 109. pp. 212-221.
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