Investigations in HPLC system containing silica dynamically modified by camphorsulfonic acid

A. Lore, Z. S. Budvári-bárány, G. Y. Szász, A. Shalaby

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Abstract

The adsorption of camphorsulfonic acid (CSA) by silica was followed by the break-through method using methanol-wat-er-CSA and acetonitril-water-CSA mixture as mobile phases. The break-through curves comprise two distinct waves, indicating the formation of CSA-double layer on the silica surface. The amount of CSA bound in the 1. and 2. layer is a function of the methanol-, acetonitril- and CSA-content of the eluent. Following the retention of a model substance along the break-through curve from methanol-water-CSA eluent, the different sorptional feature of the two CSA layers was revealed: retention increase followed by the depression of the retention could be observed before and after the first break-through paint respectively. Further study of these appearances is in progress.

Original languageEnglish
Pages (from-to)2065-2077
Number of pages13
JournalJournal of Liquid Chromatography
Volume14
Issue number11
DOIs
Publication statusPublished - Jul 1 1991

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ASJC Scopus subject areas

  • Molecular Medicine

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