The binding of radiolabelled tryptophan enantiomers to human serum albumin was investigated by ultrafiltration and by the microparticle technique. L-Trp was found to exhibit a high degree of secondary binding. D-Trp showed increased degree of binding when the HSA concentration was decreased. Stereoselective binding has also been detected in stereoselectively labelled racemic mixtures. Both L- and D-Trp were found to compete for the primary binding site with specific benzodiazepine markers. All the experiments indicate that stereoselectivity of binding is much lower than generally believed.
|Number of pages||9|
|Journal||Acta biochimica et biophysica Hungarica|
|Publication status||Published - Dec 1 1986|
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