Investigation of vinca alkaloid-plasma membrane interactions by detergent gel chromatography

T. Kremmer, L. Holczinger

Research output: Contribution to journalArticle

Abstract

Detergent gel chromatography was successfully applied for the separation of protein subunits and lipid micelles of [14C]Vincristine-treated and sodium dodecyl sulphate-solubilized liver cell plasma membranes. The elution profiles of solubilized membranes depended on the pore size of the commercial agarose and agarose-polyacrylamide gels used. It was found that most of the membrane-bound Vincristine was associated with the solubilized lipid micelles and only a very small proportion was bound to protein subunits.

Original languageEnglish
Pages (from-to)287-292
Number of pages6
JournalJournal of Chromatography A
Volume191
Issue numberC
DOIs
Publication statusPublished - Apr 18 1980

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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