Investigation of thyme (Thymus vulgaris L.) essential oil by use of the BioArena system

Ágnes Móricz, Györgyi Horváth, Péter Molnár, Béla Kocsis, Andrea Böszörményi, Éva Lemberkovics, Péter Ott

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The composition of the essential oil of Thymus vulgaris L. has been determined by GC-FID and GC-MS. Because separation of thymol, carvacrol, and linalool, components of the essential oil, was more efficient by overpressured layer chromatography (OPLC) than by conventional thin-layer chromatography (TLC), the forced flow technique was used before biological detection. All three test compounds had antibacterial effect against the phytopathogenic bacterium Pseudomonas syringae pv. maculicola, in bioautography, although in essential oil thymol was present in sufficient quantity to produce an inhibiting zone in the adsorbent layer. In BioArena investigations, when reduced glutathione as a formaldehyde (HCHO) capturer was dissolved in the cell suspension before bioautographic exposure to the essential oil, the characteristic inhibiting activity of thymol and carvacrol against Bacillus subtilis soil bacteria was reduced, whereas the presence of the HCHO precursors NG-monomethyl-L- arginine or Nε-monomethyl-L-lysine enhanced their antibacterial effect. These results suggest that HCHO and its reaction products may be involved in the antibacterial activity of thymol and carvacrol.

Original languageEnglish
Pages (from-to)406-410
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume23
Issue number6
DOIs
Publication statusPublished - Dec 1 2010

Keywords

  • Bacillus subtilis
  • BioArena
  • Bioautography
  • Formaldehyde
  • Pseudomonas syringae
  • Thymus vulgaris L.

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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