Investigation of Thr715Pro P-selectin gene polymorphism and soluble P-selectin levels in type 2 diabetes mellitus

B. Nagy, Éva Csongrádi, H. Bhattoa, I. Balogh, György Blaskó, G. Paragh, J. Kappelmayer, Miklós Káplár

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Increased levels of soluble P-selectin (sP-selectin) have been shown in a number of different disorders, e.g. diabetes mellitus (DM) and cardiovascular disease (CVD). Several studies have attempted to demonstrate the association of the most intensively examined variant of P-selectin gene polymorphism (Thr715Pro) with sP-selectin levels in healthy subjects and in CVD, but contradictory data have been reported. To clarify the effect of Pro715 allele on the sP-selectin levels in type 2 DM, we analysed this polymorphism in diabetic patients and compared these data with sP-selectin levels. Type 2 DM patients (n=119), 48 BMI-matched non diabetic individuals - consisting mostly of overweight subjects - and 57 healthy volunteers were included in the study.The Thr715Pro polymorphism was analysed by PCR-RFLP, while sP-selectin levels were measured by ELISA. Significantly elevated sP-selectin levels were found in both DM and in overweight subjects compared to healthy controls. We confirmed previous reports that in healthy Pro715 allele carriers lower sP-selectin levels could be measured; however, this difference was only significant in case of lean subjects. No significant difference was detected in sP-selectin level among DM and overweight individuals according to this genotype. However, significant difference was observed in sP-selectin levels in older DM patients compared to younger ones, but these levels were not accounted for by the Thr715Pro polymorphism. We suggest that in type 2 DM individuals, the significantly elevated sP-selectin levels are not due to the Thr715Pro P-selectin gene polymorphism.

Original languageEnglish
Pages (from-to)186-191
Number of pages6
JournalThrombosis and Haemostasis
Volume98
Issue number1
DOIs
Publication statusPublished - Jul 2007

Fingerprint

P-Selectin
Type 2 Diabetes Mellitus
Genes
Diabetes Mellitus
Healthy Volunteers
Cardiovascular Diseases
Alleles
Restriction Fragment Length Polymorphisms
Enzyme-Linked Immunosorbent Assay
Genotype

Keywords

  • Platelet activation
  • Soluble P-selectin
  • Thr715Pro P-selectin polymorphism
  • Type 2 DM

ASJC Scopus subject areas

  • Hematology

Cite this

Investigation of Thr715Pro P-selectin gene polymorphism and soluble P-selectin levels in type 2 diabetes mellitus. / Nagy, B.; Csongrádi, Éva; Bhattoa, H.; Balogh, I.; Blaskó, György; Paragh, G.; Kappelmayer, J.; Káplár, Miklós.

In: Thrombosis and Haemostasis, Vol. 98, No. 1, 07.2007, p. 186-191.

Research output: Contribution to journalArticle

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