Investigation of the toxic effect of cadmium on Candida humicola and Bacillus subtilis using a microcalorimetric method

Hai Yan Chen, Jun Yao, Yong Zhou, Hui Lun Chen, Fei Wang, Nan Gai, Ren Sheng Zhuang, Brunello Ceccanti, Thomas Maskow, G. Záray

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In this study, the technique of microcalorimetry based on heat-output by aerobic bacterial respiration was explored to evaluate the toxic effect of cadmium on Candida humicola, Bacillus subtilis, singularly or in a mixture of both. Power-time curves of the growth metabolism of C. humicola and B. subtilis and the effect of Cd2+ were studied using the TAM III (the third generation thermal activity monitor) multi-channel microcalorimetric system, isothermal mode, at 28 °C. The differences in shape of the power-time curves and the thermodynamic and kinetic characteristics of microorganisms growth were compared. The effect of cadmium added into microorganism would significantly reduce the life cycle and change the thermal effect of microbial metabolic process with different concentrations of Cd2+. The experimental results revealed that at the same concentration, the sequence of inhibitory ratio (I) and maximum thermal power (Pmax) of the Cd2+ was: mixed microorganisms > C. humicola > B. subtilis. The sequence of total thermal effect (Qtotal) and growth rate constant (k) is mixed microorganisms > B. subtilis > C. humicola. These results are important to further studies of the physiology and pharmacology of C. humicola and B. subtilis and may support the theory of restoring contaminated soil.

Original languageEnglish
Pages (from-to)465-470
Number of pages6
JournalJournal of Hazardous Materials
Volume159
Issue number2-3
DOIs
Publication statusPublished - Nov 30 2008

Fingerprint

Candida
Poisons
Bacilli
Bacillus subtilis
Cadmium
Microorganisms
cadmium
Hot Temperature
microorganism
temperature effect
Thermal effects
Growth
thermal power
Physiology
Metabolism
physiology
Life cycle
Rate constants
Life Cycle Stages
respiration

Keywords

  • Bacillus subtilis
  • Candida humicola
  • Cd
  • Microcalorimetry
  • Toxic effect

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Environmental Chemistry
  • Environmental Engineering

Cite this

Investigation of the toxic effect of cadmium on Candida humicola and Bacillus subtilis using a microcalorimetric method. / Chen, Hai Yan; Yao, Jun; Zhou, Yong; Chen, Hui Lun; Wang, Fei; Gai, Nan; Zhuang, Ren Sheng; Ceccanti, Brunello; Maskow, Thomas; Záray, G.

In: Journal of Hazardous Materials, Vol. 159, No. 2-3, 30.11.2008, p. 465-470.

Research output: Contribution to journalArticle

Chen, Hai Yan ; Yao, Jun ; Zhou, Yong ; Chen, Hui Lun ; Wang, Fei ; Gai, Nan ; Zhuang, Ren Sheng ; Ceccanti, Brunello ; Maskow, Thomas ; Záray, G. / Investigation of the toxic effect of cadmium on Candida humicola and Bacillus subtilis using a microcalorimetric method. In: Journal of Hazardous Materials. 2008 ; Vol. 159, No. 2-3. pp. 465-470.
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