Investigation of the reaction of cisplatin with methionine in aqueous media using HPLC-ICP-DRCMS

Zs Stefánka, S. Hann, G. Koellensperger, G. Stingeder

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Abstract

The reaction of cisplatin with methionine was studied by high performance ion chromatography coupled to inductively coupled plasma mass spectrometry equipped with dynamic reaction cell technique (HPLC-ICP-DRCMS) at two different cisplatin concentrations simulating chemotherapy conditions and waste water levels. The reaction of cisplatin with methionine was monitored over a period of 16 h. Accurate quantification of all platinum containing compounds was achieved via species unspecific on-line isotope dilution mass spectrometry. A limit of detection (LOD) of 0.31, 0.25, 3.83, 1.07, 0.56, 0.82 and 2.38 μg L -1 was calculated at m/z 194 for cisplatin, monoaquacisplatin, diaquacisplatin and the four platinum containing adducts, respectively. Stoichiometric platinum/sulfur ratios were assessed for characterization of the four adducts using ICP-DRCMS detection employing oxygen as reaction gas. An excellent sulfur detection limit of 1.30 μg L-1 could be achieved by HPLC-ICP-DRCMS (20 μL injection volume). At high cisplatin levels (0.6 mmol L-1) typical in chemotherapy, it was found that adducts show different kinetic behavior depending on the two investigated chloride levels (1.5 and 150 mmol L-1). Moreover, the reaction course depended on the concentration of the reactants, i.e. cisplatin and methionine. Experiments simulating possible reactions of the compounds in the aquatic environment revealed that at low μmol L-1 levels no adduct formation occurred. Finally, the stability of the four adducts potentially formed during chemotherapy was investigated representing the dilution of patient urine via hospital waste water. A considerable amount of highly active monoaquacisplatin was formed, indicating a reversal of detoxification reaction pathways of the human body.

Original languageEnglish
Pages (from-to)894-898
Number of pages5
JournalJournal of analytical atomic spectrometry
Volume19
Issue number7
DOIs
Publication statusPublished - Dec 1 2004

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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