Investigation of the pharmacokinetics of the ABCG2 transporter inhibitor Ko134 in mice by a newly developed and validated HPLC method

Anita Sztojkov-Ivanov, Orsolya Szolomajer-Csikós, Árpád Márki, Gábor K. Tóth, István Zupkó

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Transporters belonging in the ATP-binding cassette (ABC) family are crucially involved in the determination of the pharmacokinetic behavior of xenobiotics and in the development of drug resistance. The targeting of these transporters has been accepted as a rational option via which to modify the absorption or distribution of pharmaceutical agents and to combat ABC-related inefficiency. Inhibitors of the breast cancer resistance protein (BCRP/ABCG2) multidrug transporter are of interest as chemosensitizers for clinical drug resistance, for improving the pharmacokinetics of substrate chemotherapeutic drugs, and in functional assays of BCRP activity for tailoring chemotherapy. In this study, a reversephase high-performance liquid chromatographic method was developed for the determination of a simplified fumitremorgin C analog, Ko134, a potent ABCG2 inhibitor, in murine serum. The assay involves a simple sample preparation step followed by chromatographic separation on a C8 reversed-phase analytical column. The calibration plots were linear over the range 0.1 to10 μg/ml (r > 0.99). The limits of detection and quantification were 10 ng/ml and 50 ng/ml, respec- tively. The validation results demonstrated that the method is precise, accurate and selective for the determination of Ko134 in mouse serum. The method was successfully applied to evaluate the pharmacokinetic parameters of Ko134 after different modes of administration in mice.

Original languageEnglish
Pages (from-to)30-37
Number of pages8
JournalCurrent Pharmaceutical Analysis
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 14 2014

Keywords

  • Drug administration
  • HPLC
  • Ko134
  • Mouse serum
  • Pharmacokinetics
  • Validation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Medicine
  • Pharmaceutical Science

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