Investigation of the dendritic geometry of brain stem motoneurons with different functions using multivariant statistical techniques in the frog

C. Matesz, A. Birinyi, D. S. Kothalawala, G. Székely

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We give an account of an effort to make quantitative morphological distinctions between motoneurons innervating functionally different muscles in the trigeminal and facial motor nuclei of the frog. Six groups of neurons were considered in the two nuclei on the basis of their peripheral targets. One group consisted of neurons (n = 7) innervating the levator bulbi muscle, which separates the orbital cavity from the oral cavity. In the second, third and fourth groups, motoneurons (n = 27) innervating jaw closer muscles (temporalis, masseter, pterygoideus) were studied. Neurons (n = 6) innervating the submaxillary muscle comprised the fifth group. This muscle forms the muscular floor of the mouth. It is active in deglutition and contributes to the opening of the mouth. The sixth group is formed by neurons of the facial nucleus (n = 7), which innervate the depressor mandibulae muscle. This is the main opener of the mouth. Neurons were selectively stained by cobalt labelling through the muscle nerves and the morphometric values of successfully labelled neurons were fed into a IBM AT 386 computer through a digitizing tablet for three-dimensional reconstruction. Four neurons labelled directly through the motor root of the trigeminal nerve but innervating unidentified muscles were added to the investigation. Two sets of quantitative measurements were taken from the neurons. In the first set (neurometric data), 17 quantitative variables were measured in the perikaryon and the dendritic arbor. In the second set, 15 variables concerned with the orientation and shape of the dendritic tree, the relation of the perikaryon to the dendritic tree and the spatial expansion of dendrites were measured in the three dimensions of Cartesian space (product-moment data). The data were subjected to multivariant statistical analysis. First, they were partitioned with cluster analysis. The average linkage between groups algorithm and the cosine of vectors of variables, or the Pearson correlation similarity coefficients were used. Neurometric data and product-moment data were analysed separately and in combination, and six to seven clusters were considered. In each case, the majority of neurons innervating jaw closer muscles were grouped into clusters different from neurons innervating jaw opener muscles. The best separation of functionally different neurons was achieved with the neurometric data set. The groups of neurons obtained from cluster analysis were subjected to non-parametric discriminant analysis with the eight nearest-neighbour classification criterion, and the results were checked with a cross-validation technique. The results of cluster analysis performed with the combined neurometric and product-moment data sets were 100% verified with high average posterior probabilities. With the neurometric data set two neurons and with the product-moment data set three neurons were found to be erroneously classified. It was concluded that multivariant statistical techniques were capable of distinguishing functionally different neurons on the basis of their quantitative parameters describing the size and shape of the dendritic tree. The possibility is proposed that the characteristic geometry of the dendritic tree, placed within a given synaptic field, may have a preference for one array of fibres over another and this plays a role in the establishment of appropriate connections with the corresponding premotor structures.

Original languageEnglish
Pages (from-to)1129-1144
Number of pages16
JournalNeuroscience
Volume65
Issue number4
DOIs
Publication statusPublished - 1995

Fingerprint

Motor Neurons
Anura
Brain Stem
Neurons
Muscles
Jaw
Cluster Analysis
Mouth
Mouth Floor
Masseter Muscle
Trigeminal Nerve
Discriminant Analysis
Deglutition
Dendrites
Cobalt
Tablets

ASJC Scopus subject areas

  • Neuroscience(all)

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Investigation of the dendritic geometry of brain stem motoneurons with different functions using multivariant statistical techniques in the frog. / Matesz, C.; Birinyi, A.; Kothalawala, D. S.; Székely, G.

In: Neuroscience, Vol. 65, No. 4, 1995, p. 1129-1144.

Research output: Contribution to journalArticle

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