Investigation of the carrier function of polypeptides. Synthesis, conformation and cytotoxicity of oxazolone conjugates of poly(Lys) and poly[Lys‐(DL‐ALAm)]

F. Hudecz, Judit Kajtȧr, IstvȧN Kurucz, Attila L. Kovȧcs, MȧRia Szekerke

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Abstract

A new group of synthetic macromolecular conjugates was synthesised in which poly(Lys) or a branched polypeptide poly[Lys‐(DL‐Alam)] (m≅3) were used as carrier and 4‐(ethoxymethylene)‐2–phenyl–5 (4H)‐oxazolone as hapten. These conjugates were characterized by amino acid analysis, identification of terminal residues of the side chain, sedimentation analysis. The conformation of conjugates and of carrier polypeptides were analyzed by circular dichroism spectroscopy in water solutions at various pH and ionic strengths. These data indicated a marked dependence of the conformation of the conjugate on the structure of carrier and on the number of the side chain terminal haptens (phOx). In vitro cytotoxicity of these polymers was also investigated using two different assays, by measuring the viability of isolated rat liver cells and the effect on growth of HeLa cells. Toxicity of polypeptides could be diminished by conjugates containing a higher amount of oxazolone. These conjugates with defined conformation and toxicological properties are considered suitable to analyse the carrier function of branched polypeptides, particularly the interaction with the immunological network.

Original languageEnglish
Pages (from-to)107-124
Number of pages18
JournalMakromolekulare Chemie. Macromolecular Symposia
Volume19
Issue number1
DOIs
Publication statusPublished - 1988

Fingerprint

Oxazolone
Polypeptides
polypeptides
Cytotoxicity
Conformations
Peptides
Haptens
synthesis
Circular dichroism spectroscopy
Ionic strength
Sedimentation
Liver
Toxicity
Amino acids
Rats
Assays
Polymers
cells
viability
liver

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

Cite this

Investigation of the carrier function of polypeptides. Synthesis, conformation and cytotoxicity of oxazolone conjugates of poly(Lys) and poly[Lys‐(DL‐ALAm)]. / Hudecz, F.; Kajtȧr, Judit; Kurucz, IstvȧN; Kovȧcs, Attila L.; Szekerke, MȧRia.

In: Makromolekulare Chemie. Macromolecular Symposia, Vol. 19, No. 1, 1988, p. 107-124.

Research output: Contribution to journalArticle

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