T-Limfociták aktivációjának vizsgálata t-sejt-receptor variábilis bétalánc profil elemzésével normál placentában és mola terhességben

Translated title of the contribution: Investigation of t-lym- Phocyte activation with variable beta chain profile ana-lysis of T-cell receptor in normal placenta and molar pregnancy

Nagymányoki Zoltán, Michael J. Callahan, Mana M. Parast, Ross S. Berkowitz, Samuel C. Mok, V. Fülöp

Research output: Contribution to journalArticle

Abstract

Objeczive: It is reported that complete molar pregnancy induces a more vigorous local T-cell response than that seen in normal pregnancy. However, the activating antigen is still unknown. Previous investigators have used T-cell-re- ceptor (TCR) variable beta chain (VBC) analysis in several autoimune diseases like rheumatoid arthritis and Hashimoto's thyroiditis to identify specific antigen responses against different tissues. The present study was undertaken to investigate the antigenicity of normal placenta and complete molar trophoblastic tissue. METHODS: TCR VBC gene expression was analyzed utilizing fresh frozen tissues. Real-time polymerase chain reactions (RT-PCR) were performed on cDNA samples from 10 normal buffy coats (BC), 7 normal placentas (NP), and 14 complete molar pregnancies (CM) using a TCR beta chain and 25 variable TCR beta chain primers. Relative expressions were calculated for each individual gene. Results: Signficant changes were noted in most of the gene expressions in NP and CM compared to the buffy coat samples. The relative expression of most genes was significantly decreased in NP and CM, but VBC gene number 4 was increased in both NP and CM, however a significant difference was noted only between BC and CM (p=0.023). Comparing NP to CM, five other variable beta chain genes expression were decreased significantly in the complete molar tissues (p

Original languageHungarian
Pages (from-to)19-26
Number of pages8
JournalMagyar Noorvosok Lapja
Volume72
Issue number1
Publication statusPublished - 2009

Fingerprint

Hydatidiform Mole
Lymphocyte Activation
T-Cell Antigen Receptor
Placenta
Antigen Receptors, T-Cell, alpha-beta
Gene Expression
Antigens
Hashimoto Disease
Genes
Real-Time Polymerase Chain Reaction
Rheumatoid Arthritis
Complementary DNA
Research Personnel
T-Lymphocytes
Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

T-Limfociták aktivációjának vizsgálata t-sejt-receptor variábilis bétalánc profil elemzésével normál placentában és mola terhességben. / Zoltán, Nagymányoki; Callahan, Michael J.; Parast, Mana M.; Berkowitz, Ross S.; Mok, Samuel C.; Fülöp, V.

In: Magyar Noorvosok Lapja, Vol. 72, No. 1, 2009, p. 19-26.

Research output: Contribution to journalArticle

Zoltán, Nagymányoki ; Callahan, Michael J. ; Parast, Mana M. ; Berkowitz, Ross S. ; Mok, Samuel C. ; Fülöp, V. / T-Limfociták aktivációjának vizsgálata t-sejt-receptor variábilis bétalánc profil elemzésével normál placentában és mola terhességben. In: Magyar Noorvosok Lapja. 2009 ; Vol. 72, No. 1. pp. 19-26.
@article{8f103579d0384260b46bde14fb02f61a,
title = "T-Limfocit{\'a}k aktiv{\'a}ci{\'o}j{\'a}nak vizsg{\'a}lata t-sejt-receptor vari{\'a}bilis b{\'e}tal{\'a}nc profil elemz{\'e}s{\'e}vel norm{\'a}l placent{\'a}ban {\'e}s mola terhess{\'e}gben",
abstract = "Objeczive: It is reported that complete molar pregnancy induces a more vigorous local T-cell response than that seen in normal pregnancy. However, the activating antigen is still unknown. Previous investigators have used T-cell-re- ceptor (TCR) variable beta chain (VBC) analysis in several autoimune diseases like rheumatoid arthritis and Hashimoto's thyroiditis to identify specific antigen responses against different tissues. The present study was undertaken to investigate the antigenicity of normal placenta and complete molar trophoblastic tissue. METHODS: TCR VBC gene expression was analyzed utilizing fresh frozen tissues. Real-time polymerase chain reactions (RT-PCR) were performed on cDNA samples from 10 normal buffy coats (BC), 7 normal placentas (NP), and 14 complete molar pregnancies (CM) using a TCR beta chain and 25 variable TCR beta chain primers. Relative expressions were calculated for each individual gene. Results: Signficant changes were noted in most of the gene expressions in NP and CM compared to the buffy coat samples. The relative expression of most genes was significantly decreased in NP and CM, but VBC gene number 4 was increased in both NP and CM, however a significant difference was noted only between BC and CM (p=0.023). Comparing NP to CM, five other variable beta chain genes expression were decreased significantly in the complete molar tissues (p",
keywords = "Gestational trophoblastic disease, Lymphocyte activation, T-cell-receptor, T-lym- Phocytes",
author = "Nagym{\'a}nyoki Zolt{\'a}n and Callahan, {Michael J.} and Parast, {Mana M.} and Berkowitz, {Ross S.} and Mok, {Samuel C.} and V. F{\"u}l{\"o}p",
year = "2009",
language = "Hungarian",
volume = "72",
pages = "19--26",
journal = "Magyar Noorvosok Lapja",
issn = "0025-021X",
publisher = "Magyar Noovos Tarsasag",
number = "1",

}

TY - JOUR

T1 - T-Limfociták aktivációjának vizsgálata t-sejt-receptor variábilis bétalánc profil elemzésével normál placentában és mola terhességben

AU - Zoltán, Nagymányoki

AU - Callahan, Michael J.

AU - Parast, Mana M.

AU - Berkowitz, Ross S.

AU - Mok, Samuel C.

AU - Fülöp, V.

PY - 2009

Y1 - 2009

N2 - Objeczive: It is reported that complete molar pregnancy induces a more vigorous local T-cell response than that seen in normal pregnancy. However, the activating antigen is still unknown. Previous investigators have used T-cell-re- ceptor (TCR) variable beta chain (VBC) analysis in several autoimune diseases like rheumatoid arthritis and Hashimoto's thyroiditis to identify specific antigen responses against different tissues. The present study was undertaken to investigate the antigenicity of normal placenta and complete molar trophoblastic tissue. METHODS: TCR VBC gene expression was analyzed utilizing fresh frozen tissues. Real-time polymerase chain reactions (RT-PCR) were performed on cDNA samples from 10 normal buffy coats (BC), 7 normal placentas (NP), and 14 complete molar pregnancies (CM) using a TCR beta chain and 25 variable TCR beta chain primers. Relative expressions were calculated for each individual gene. Results: Signficant changes were noted in most of the gene expressions in NP and CM compared to the buffy coat samples. The relative expression of most genes was significantly decreased in NP and CM, but VBC gene number 4 was increased in both NP and CM, however a significant difference was noted only between BC and CM (p=0.023). Comparing NP to CM, five other variable beta chain genes expression were decreased significantly in the complete molar tissues (p

AB - Objeczive: It is reported that complete molar pregnancy induces a more vigorous local T-cell response than that seen in normal pregnancy. However, the activating antigen is still unknown. Previous investigators have used T-cell-re- ceptor (TCR) variable beta chain (VBC) analysis in several autoimune diseases like rheumatoid arthritis and Hashimoto's thyroiditis to identify specific antigen responses against different tissues. The present study was undertaken to investigate the antigenicity of normal placenta and complete molar trophoblastic tissue. METHODS: TCR VBC gene expression was analyzed utilizing fresh frozen tissues. Real-time polymerase chain reactions (RT-PCR) were performed on cDNA samples from 10 normal buffy coats (BC), 7 normal placentas (NP), and 14 complete molar pregnancies (CM) using a TCR beta chain and 25 variable TCR beta chain primers. Relative expressions were calculated for each individual gene. Results: Signficant changes were noted in most of the gene expressions in NP and CM compared to the buffy coat samples. The relative expression of most genes was significantly decreased in NP and CM, but VBC gene number 4 was increased in both NP and CM, however a significant difference was noted only between BC and CM (p=0.023). Comparing NP to CM, five other variable beta chain genes expression were decreased significantly in the complete molar tissues (p

KW - Gestational trophoblastic disease

KW - Lymphocyte activation

KW - T-cell-receptor

KW - T-lym- Phocytes

UR - http://www.scopus.com/inward/record.url?scp=62249221311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62249221311&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:62249221311

VL - 72

SP - 19

EP - 26

JO - Magyar Noorvosok Lapja

JF - Magyar Noorvosok Lapja

SN - 0025-021X

IS - 1

ER -