Objeczive: It is reported that complete molar pregnancy induces a more vigorous local T-cell response than that seen in normal pregnancy. However, the activating antigen is still unknown. Previous investigators have used T-cell-re- ceptor (TCR) variable beta chain (VBC) analysis in several autoimune diseases like rheumatoid arthritis and Hashimoto's thyroiditis to identify specific antigen responses against different tissues. The present study was undertaken to investigate the antigenicity of normal placenta and complete molar trophoblastic tissue. METHODS: TCR VBC gene expression was analyzed utilizing fresh frozen tissues. Real-time polymerase chain reactions (RT-PCR) were performed on cDNA samples from 10 normal buffy coats (BC), 7 normal placentas (NP), and 14 complete molar pregnancies (CM) using a TCR beta chain and 25 variable TCR beta chain primers. Relative expressions were calculated for each individual gene. Results: Signficant changes were noted in most of the gene expressions in NP and CM compared to the buffy coat samples. The relative expression of most genes was significantly decreased in NP and CM, but VBC gene number 4 was increased in both NP and CM, however a significant difference was noted only between BC and CM (p=0.023). Comparing NP to CM, five other variable beta chain genes expression were decreased significantly in the complete molar tissues (p<0.05). Conclusion: In normal placenta and complete molar pregnancies, T-cells appear to express certain TCR VBC genes in a different manner than in BC. These genes and the VBC gene profile difference found between NP and CM may have an important role in the immunobiology of complete molar pregnancy.
|Number of pages||8|
|Journal||Magyar Noorvosok Lapja|
|Publication status||Published - Mar 19 2009|
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynaecology