Investigation of some methylated compounds and peroxidase activity during plant ontogenesis in snap bean

Research output: Contribution to journalArticle

Abstract

Changes in the level of endogenous formaldehyde (HCHO) and some N-methylated compounds were investigated in the leaves of snap bean (Phaseolus vulgaris L.) during ontogenesis. In addition, the activity and isozyme pattern of peroxidase enzymes were also examined. HCHO, as dimedone adduct, and fully N-methylated compounds were determined by overpressured layer chromatography in different development stages of snap bean plant. Peroxidase activities were measured by spectrophotometry and isozyme patterns were examined by isoelectric focusing. HCHO level decreased until blooming with aging of the plant being the highest in the youngest leaves all the time, but then increased again in old leaf tissues. At the same time the concentration of choline and trigonelline as potential HCHO generators (marker molecules or gene products) decreased considerably while peroxidase activity increased with aging of plants.

Original languageEnglish
Pages (from-to)381-391
Number of pages11
JournalActa Biologica Hungarica
Volume49
Issue number2-4
Publication statusPublished - 1998

Fingerprint

green beans
Peroxidase
ontogeny
peroxidase
Isoenzymes
isozymes
Aging of materials
trigonelline
leaves
Phaseolus
Spectrophotometry
isoelectric focusing
Isoelectric Focusing
choline
spectrophotometry
Chromatography
Choline
formaldehyde
Formaldehyde
Phaseolus vulgaris

Keywords

  • Demethylation
  • Fully N-methylated compounds
  • Methylation
  • Ontogenesis
  • Peroxidase activity
  • Snap bean

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Investigation of some methylated compounds and peroxidase activity during plant ontogenesis in snap bean. / Sárdi, Éva; Stefanovits-Banyai, E.

In: Acta Biologica Hungarica, Vol. 49, No. 2-4, 1998, p. 381-391.

Research output: Contribution to journalArticle

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