Investigation of polyphenol resistance of aspergillus flavus on cornmeal media

Szilvia Kovács, T. Pusztahelyi

Research output: Contribution to journalArticle

Abstract

Cornmeal agar (CMA) is a good to model natural conditions (low C and N, high antioxidants, crude fat) for phytopathogenic fungi. Different CMA media was prepared to model the maize kernel as growth environment for Aspergillus flavus, where stress resistance and aflatoxin B1 (AFB1) production were tested. The CMA medium with high polyphenol and low fatty acid content did not support the mycelial growth and high AFB1 production but the sclerotia development of the cultures. High fatty acid content in the CMA exceeded the inhibitory effect of antioxidant polyphenols of corn and low concentration of AFB1 was detected. Glucose supplement of CMA induced AFB1 production proving the need for free carbon source for the secondary metabolite pathway. The tolerance of the fungus against salt and cell membrane stress was lowered on CMA. At higher fatty acid concentration, the aflatoxin B1 production cannot be hindered by the natural antioxidants and that is important in selection of resistant corn hybrids.

Original languageEnglish
Pages (from-to)143-162
Number of pages20
JournalActa Phytopathologica et Entomologica Hungarica
Volume53
Issue number2
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

corn meal
Aspergillus flavus
aflatoxin B1
polyphenols
agar
antioxidants
corn
fatty acid composition
sclerotia
plant pathogenic fungi
stress tolerance
secondary metabolites
cell membranes
salts
fatty acids
glucose
fungi
carbon
lipids
seeds

Keywords

  • Aflatoxin B1
  • Aflatoxin B1; MA
  • Aspergillus flavus
  • Cornmeal agar
  • Cornmeal. Abbreviations: AFB1
  • Flavonoids
  • Malate agar; CMA
  • Polyphenol
  • Stress tolerance

ASJC Scopus subject areas

  • Plant Science
  • Insect Science

Cite this

Investigation of polyphenol resistance of aspergillus flavus on cornmeal media. / Kovács, Szilvia; Pusztahelyi, T.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 53, No. 2, 01.12.2018, p. 143-162.

Research output: Contribution to journalArticle

@article{8b4835afc5e94a9db75683992575b94c,
title = "Investigation of polyphenol resistance of aspergillus flavus on cornmeal media",
abstract = "Cornmeal agar (CMA) is a good to model natural conditions (low C and N, high antioxidants, crude fat) for phytopathogenic fungi. Different CMA media was prepared to model the maize kernel as growth environment for Aspergillus flavus, where stress resistance and aflatoxin B1 (AFB1) production were tested. The CMA medium with high polyphenol and low fatty acid content did not support the mycelial growth and high AFB1 production but the sclerotia development of the cultures. High fatty acid content in the CMA exceeded the inhibitory effect of antioxidant polyphenols of corn and low concentration of AFB1 was detected. Glucose supplement of CMA induced AFB1 production proving the need for free carbon source for the secondary metabolite pathway. The tolerance of the fungus against salt and cell membrane stress was lowered on CMA. At higher fatty acid concentration, the aflatoxin B1 production cannot be hindered by the natural antioxidants and that is important in selection of resistant corn hybrids.",
keywords = "Aflatoxin B1, Aflatoxin B1; MA, Aspergillus flavus, Cornmeal agar, Cornmeal. Abbreviations: AFB1, Flavonoids, Malate agar; CMA, Polyphenol, Stress tolerance",
author = "Szilvia Kov{\'a}cs and T. Pusztahelyi",
year = "2018",
month = "12",
day = "1",
doi = "10.1556/038.53.2018.009",
language = "English",
volume = "53",
pages = "143--162",
journal = "Acta Phytopathologica et Entomologica Hungarica",
issn = "0238-1249",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - Investigation of polyphenol resistance of aspergillus flavus on cornmeal media

AU - Kovács, Szilvia

AU - Pusztahelyi, T.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Cornmeal agar (CMA) is a good to model natural conditions (low C and N, high antioxidants, crude fat) for phytopathogenic fungi. Different CMA media was prepared to model the maize kernel as growth environment for Aspergillus flavus, where stress resistance and aflatoxin B1 (AFB1) production were tested. The CMA medium with high polyphenol and low fatty acid content did not support the mycelial growth and high AFB1 production but the sclerotia development of the cultures. High fatty acid content in the CMA exceeded the inhibitory effect of antioxidant polyphenols of corn and low concentration of AFB1 was detected. Glucose supplement of CMA induced AFB1 production proving the need for free carbon source for the secondary metabolite pathway. The tolerance of the fungus against salt and cell membrane stress was lowered on CMA. At higher fatty acid concentration, the aflatoxin B1 production cannot be hindered by the natural antioxidants and that is important in selection of resistant corn hybrids.

AB - Cornmeal agar (CMA) is a good to model natural conditions (low C and N, high antioxidants, crude fat) for phytopathogenic fungi. Different CMA media was prepared to model the maize kernel as growth environment for Aspergillus flavus, where stress resistance and aflatoxin B1 (AFB1) production were tested. The CMA medium with high polyphenol and low fatty acid content did not support the mycelial growth and high AFB1 production but the sclerotia development of the cultures. High fatty acid content in the CMA exceeded the inhibitory effect of antioxidant polyphenols of corn and low concentration of AFB1 was detected. Glucose supplement of CMA induced AFB1 production proving the need for free carbon source for the secondary metabolite pathway. The tolerance of the fungus against salt and cell membrane stress was lowered on CMA. At higher fatty acid concentration, the aflatoxin B1 production cannot be hindered by the natural antioxidants and that is important in selection of resistant corn hybrids.

KW - Aflatoxin B1

KW - Aflatoxin B1; MA

KW - Aspergillus flavus

KW - Cornmeal agar

KW - Cornmeal. Abbreviations: AFB1

KW - Flavonoids

KW - Malate agar; CMA

KW - Polyphenol

KW - Stress tolerance

UR - http://www.scopus.com/inward/record.url?scp=85060026826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060026826&partnerID=8YFLogxK

U2 - 10.1556/038.53.2018.009

DO - 10.1556/038.53.2018.009

M3 - Article

AN - SCOPUS:85060026826

VL - 53

SP - 143

EP - 162

JO - Acta Phytopathologica et Entomologica Hungarica

JF - Acta Phytopathologica et Entomologica Hungarica

SN - 0238-1249

IS - 2

ER -