Thin-layer chromatography has been used to monitor formaldehyde production during in-vivo metabolism of (-)-deprenyl. Formaldehyde was reacted with dimedone to yield its dimedone adduct, formaldemedone. Determination of 14C-labeled formaldemedone was performed by planar displacement chromatography. The separated bands were detected either by digital autoradiography or on X-ray film after contact autoradiography. Two-dimensional separations have improved the evaluation.
- Thin-layer chromatography-digital autoradiography
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry