Investigation of metabolism using TLC-DAR and reaction-displacement TLC

Huba Kalász, József Lengyel, Tibor Szarvas, György Morovján, Imre Klebovich

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Thin-layer chromatography has been used to monitor formaldehyde production during in-vivo metabolism of (-)-deprenyl. Formaldehyde was reacted with dimedone to yield its dimedone adduct, formaldemedone. Determination of 14C-labeled formaldemedone was performed by planar displacement chromatography. The separated bands were detected either by digital autoradiography or on X-ray film after contact autoradiography. Two-dimensional separations have improved the evaluation.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume16
Issue number5
DOIs
Publication statusPublished - Sep 1 2003

Keywords

  • (-)-deprenyl
  • Formaldehyde
  • Formaldemedone
  • Metabolism
  • N-demethylation
  • Thin-layer chromatography-digital autoradiography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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