Investigation of iron pools in cucumber roots by Mössbauer spectroscopy

Direct evidence for the Strategy I iron uptake mechanism

Krisztina Kovács, E. Kuzmann, E. Tatár, A. Vértes, F. Fodor

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Distinct chemical species of iron were investigated by Mössbauer spectroscopy during iron uptake into cucumber roots grown in unbuffered nutrient solution with or without 57Fe-citrate. Mössbauer spectra of iron deficient roots supplied with 10-500 μM 57Fe-citrate for 30-180 min and 24 h and iron-sufficient ones, were recorded. The roots were analysed for Fe concentration and Fe reductase activity. The Mössbauer parameters in the case of iron-sufficient roots revealed high-spin iron(III) components suggesting the presence of FeIII-carboxylate complexes, hydrous ferric oxides and sulfate-hydroxide containing species. No Fe II was detected in these roots. However, iron-deficient roots supplied with 0.5 mM 57FeIII-citrate for 30 min contained significant amount of FeII in a hexaaqua complex form. This is a direct evidence for the Strategy I iron uptake mechanism. Correlation was found between the decrease in Fe reductase activity and the ratio of Fe II-FeIII components as the time of iron supply was increased. The data may refer to a higher iron reduction rate as compared to its uptake/reoxidation in the cytoplasm in accordance with the increased reduction rate in iron deficient Strategy I plants.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalPlanta
Volume229
Issue number2
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Cucumis sativus
cucumbers
spectroscopy
Spectrum Analysis
Iron
iron
uptake mechanisms
Citric Acid
citrates
Oxidoreductases
ferric sulfate
chemical speciation
hydroxides
nutrient solutions
Cytoplasm
cytoplasm

Keywords

  • Cucumis sativus (cucumber)
  • Fe reductase activity
  • Fe-citrate
  • Mössbauer spectroscopy
  • Strategy I

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

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title = "Investigation of iron pools in cucumber roots by M{\"o}ssbauer spectroscopy: Direct evidence for the Strategy I iron uptake mechanism",
abstract = "Distinct chemical species of iron were investigated by M{\"o}ssbauer spectroscopy during iron uptake into cucumber roots grown in unbuffered nutrient solution with or without 57Fe-citrate. M{\"o}ssbauer spectra of iron deficient roots supplied with 10-500 μM 57Fe-citrate for 30-180 min and 24 h and iron-sufficient ones, were recorded. The roots were analysed for Fe concentration and Fe reductase activity. The M{\"o}ssbauer parameters in the case of iron-sufficient roots revealed high-spin iron(III) components suggesting the presence of FeIII-carboxylate complexes, hydrous ferric oxides and sulfate-hydroxide containing species. No Fe II was detected in these roots. However, iron-deficient roots supplied with 0.5 mM 57FeIII-citrate for 30 min contained significant amount of FeII in a hexaaqua complex form. This is a direct evidence for the Strategy I iron uptake mechanism. Correlation was found between the decrease in Fe reductase activity and the ratio of Fe II-FeIII components as the time of iron supply was increased. The data may refer to a higher iron reduction rate as compared to its uptake/reoxidation in the cytoplasm in accordance with the increased reduction rate in iron deficient Strategy I plants.",
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AU - Kovács, Krisztina

AU - Kuzmann, E.

AU - Tatár, E.

AU - Vértes, A.

AU - Fodor, F.

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AB - Distinct chemical species of iron were investigated by Mössbauer spectroscopy during iron uptake into cucumber roots grown in unbuffered nutrient solution with or without 57Fe-citrate. Mössbauer spectra of iron deficient roots supplied with 10-500 μM 57Fe-citrate for 30-180 min and 24 h and iron-sufficient ones, were recorded. The roots were analysed for Fe concentration and Fe reductase activity. The Mössbauer parameters in the case of iron-sufficient roots revealed high-spin iron(III) components suggesting the presence of FeIII-carboxylate complexes, hydrous ferric oxides and sulfate-hydroxide containing species. No Fe II was detected in these roots. However, iron-deficient roots supplied with 0.5 mM 57FeIII-citrate for 30 min contained significant amount of FeII in a hexaaqua complex form. This is a direct evidence for the Strategy I iron uptake mechanism. Correlation was found between the decrease in Fe reductase activity and the ratio of Fe II-FeIII components as the time of iron supply was increased. The data may refer to a higher iron reduction rate as compared to its uptake/reoxidation in the cytoplasm in accordance with the increased reduction rate in iron deficient Strategy I plants.

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