Investigation of iron pools in cucumber roots by Mössbauer spectroscopy: Direct evidence for the Strategy I iron uptake mechanism

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Abstract

Distinct chemical species of iron were investigated by Mössbauer spectroscopy during iron uptake into cucumber roots grown in unbuffered nutrient solution with or without 57Fe-citrate. Mössbauer spectra of iron deficient roots supplied with 10-500 μM 57Fe-citrate for 30-180 min and 24 h and iron-sufficient ones, were recorded. The roots were analysed for Fe concentration and Fe reductase activity. The Mössbauer parameters in the case of iron-sufficient roots revealed high-spin iron(III) components suggesting the presence of FeIII-carboxylate complexes, hydrous ferric oxides and sulfate-hydroxide containing species. No Fe II was detected in these roots. However, iron-deficient roots supplied with 0.5 mM 57FeIII-citrate for 30 min contained significant amount of FeII in a hexaaqua complex form. This is a direct evidence for the Strategy I iron uptake mechanism. Correlation was found between the decrease in Fe reductase activity and the ratio of Fe II-FeIII components as the time of iron supply was increased. The data may refer to a higher iron reduction rate as compared to its uptake/reoxidation in the cytoplasm in accordance with the increased reduction rate in iron deficient Strategy I plants.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalPlanta
Volume229
Issue number2
DOIs
Publication statusPublished - Jan 1 2009

Keywords

  • Cucumis sativus (cucumber)
  • Fe reductase activity
  • Fe-citrate
  • Mössbauer spectroscopy
  • Strategy I

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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