Investigation of image properties in super-resolution microscopy using two-color fluorescence dip spectroscopy

Yoshinori Iketaki, Takeshi Watanabe, Nándor Bokor, Masaaki Fujii

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We quantitatively investigated image properties in super-resolution microscopy using two-color fluorescence dip spectroscopy. To evaluate the properties, the point spread function (PSF) and contrast transfer function (CTF) were measured using a fluorescent scale together with a fluorescent bead. From the CTF, it has been found that visible light can resolve a 100 nm line-and-space pattern by microcopy, and provide a contrast of 10%. The CTF corresponds to a PSF with a FWHM of 130 nm. The value is two times finer than the diffraction limit size. An evaluation using a 100 nm φ fluorescent bead consistently supports the result given by the CTF for super-resolution microscopy. The measured CTF shows that super-resolution microscopy can indeed improve the optical properties of fluorescent images and enable us to observe a structure with a spatial resolution overcoming the diffraction limit.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XIV
DOIs
Publication statusPublished - Apr 30 2007
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV - San Jose, CA, United States
Duration: Jan 23 2007Jan 25 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6443
ISSN (Print)1605-7422

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
CountryUnited States
CitySan Jose, CA
Period1/23/071/25/07

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Keywords

  • Contrast transfer function
  • Fluorescence-depletion process
  • Point spread function
  • Rhodamine 6G
  • Super-resolution microscopy
  • Two-color dip spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Iketaki, Y., Watanabe, T., Bokor, N., & Fujii, M. (2007). Investigation of image properties in super-resolution microscopy using two-color fluorescence dip spectroscopy. In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV [644307] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6443). https://doi.org/10.1117/12.698451