New herbicides with great effectiveness, a wide spectrum and rapid decomposition often have an adverse effect the crop. The best way to achieve safe herbicide application is to select mutant lines/hybrids. The imidazolinone concentration required in the selective medium was determined for the detection of resistant cell lines originating from homozygous genotypes produced by irradiation at the Department of Plant Genetics and Breeding, Center for Agricultural Sciences, University of Debrecen. Nowadays imidazolinone-resistant inbred lines with favourable agronomic attributes can be selected by exploiting somaclonal variation in callus culture. In the course of two-step in vivo selection maize lines were selected using the 1% concentration recommended for imidazolinone herbicides (4 1/ha ESCORT™). Based on their herbicide response five inbred lines (176/F, 241/F, 271/F, 378/F, 416/F) were selected for callus induction. The best medium for the in vitro multiplication and differentiation of plant cells was MS (Murashige-Skoog) supplemented with 12.5 μM·1-1 2,4-D (2,4 dichlorphenoxy-aceticacid). When 0.1% imidazolinone was added to the medium the callus weight did not show any increase because of the sensitivity of lines. At a concentration of 0.01% imidazolinone the calli of lines 176/F and 378/F did not grow, but the callus weight of 271/F and 416/F increased by 14.41% and 16.61% respectively, while 241/F line showed nearly the same weight increase as the control. The in vitro experiments were repeated with 0.012% imidazolinone and it was confirmed that lines 176/F and 378/F were sensitive to imidazolinone. The fresh callus weight of lines 271/F and 416/F decreased as the imidazolinone concentration increased (9.75% and 10.67% of the control), while inbred line 241/F again showed the same weight increase as the control. It can be concluded that lines 271/F and 416/F are tolerant of imidazolinone, while line 241/F is resistant.
|Translated title of the contribution||Investigation of herbicide resistance of maize (Zea mays L.) inbred lines by integrating in vivo and in vitro methods|
|Number of pages||14|
|Publication status||Published - Nov 2 2006|
ASJC Scopus subject areas
- Agronomy and Crop Science