Investigation of heat-treated cultivation medium for mammalian cells with near infrared spectroscopy

Éva Szabó, László Párta, Dénes Zalai, Szilveszter Gergely, A. Salgó

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A chemically defined medium powder for Chinese hamster ovary (CHO) cell cultivation was investigated regarding its response to heat treatments with different exposure times (1 hour, 7 hours and 13 hours) and temperatures (30 °C, 50 °C and 70 °C). The heat treatments were performed according to a design of experiments approach. Spectra of the control and the treated powders were collected to compare the sample groups using a dispersive and a Fourier transform (FT) near infrared (NIR) spectrometer. Multivariate data analysis including unsupervised classification as principal component analysis and supervised classification as soft independent modelling of class analogy was employed to identify the treatment-induced variations in the samples. Samples were separated according to the temperature set points of heat treatments and the control samples were successfully discriminated based on second-derivative NIR spectra. In order to evaluate the impact of the treatments on the growth rate of CHO cells, shake flask cultivation experiments were also performed. The viable cell density results showed that both treatment temperature and treatment duration were significant factors (p = 0.05). Additionally, the sensitivity of change detection for medium powders was higher in the case of NIR and FT-NIR measurement and spectrum analysis compared to traditional shake flask cultivation.

Original languageEnglish
Pages (from-to)373-380
Number of pages8
JournalJournal of Near Infrared Spectroscopy
Volume24
Issue number4
DOIs
Publication statusPublished - 2016

Fingerprint

Near infrared spectroscopy
Powders
Heat treatment
Cells
Infrared radiation
Fourier transforms
Infrared spectrometers
Design of experiments
Principal component analysis
Spectrum analysis
Temperature
Derivatives
Hot Temperature
Experiments

Keywords

  • Chinese hamster ovary cell line (CHO) cultivation
  • Design of experiments (DoE)
  • Medium powder
  • Near infrared (NIR) spectroscopy
  • Principal component analysis (PCA)
  • Process analytical technology (PAT)
  • Qualityby design (QbD)
  • Soft independent modelling of class analogy (SIMCA)

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Investigation of heat-treated cultivation medium for mammalian cells with near infrared spectroscopy. / Szabó, Éva; Párta, László; Zalai, Dénes; Gergely, Szilveszter; Salgó, A.

In: Journal of Near Infrared Spectroscopy, Vol. 24, No. 4, 2016, p. 373-380.

Research output: Contribution to journalArticle

Szabó, Éva ; Párta, László ; Zalai, Dénes ; Gergely, Szilveszter ; Salgó, A. / Investigation of heat-treated cultivation medium for mammalian cells with near infrared spectroscopy. In: Journal of Near Infrared Spectroscopy. 2016 ; Vol. 24, No. 4. pp. 373-380.
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