A szérum zsírsavprofil diagnosztikus jelentösé gének vizsgálata kísérletes modellben

Translated title of the contribution: Investigation of fatty acid profile for diagnostic purposes in mouse melanoma

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate whether fatty acid profile is a suitable marker for diagnostic purposes in mouse melanoma. Twelve C57Bl/6 male mice were implanted with B16 mouse melanoma cells (106 cells/animal) orthotopically (subcutaneously). After the implantation 4-4 animals were bled by cardiac puncture following narcosis, at days 7, 14, and 21. In order to investigate fatty acid profiles a method based on extraction and HPLC-MS was developed. Signal intensities of 14 fatty acids were determined by mass spectrometry in tumor-free animals as well as tumor bearing animals at the three time points. Mathematical analysis showed non-significant profile changes when control (tumor-free) animals were compared to tumor-implanted ones as well as during tumor progression on week 1, 2 and 3. In case of three fatty acids (myristic acid, palmitoleic acid and eicosadienoic acid) a trend was observed during tumor progression but its statistical significance cannot be evaluated without further investigations. The fatty acid profile cannot be used for early diagnoses in mouse melanoma.

Translated title of the contributionInvestigation of fatty acid profile for diagnostic purposes in mouse melanoma
Original languageHungarian
Pages (from-to)199-204
Number of pages6
JournalMagyar onkologia
Volume55
Issue number3
Publication statusPublished - 2011

ASJC Scopus subject areas

  • Medicine(all)

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