Investigation of Euphrasia rostkoviana Hayne using GC-MS and LC-MS

Balazs Blazics, K. Ludányi, Szabolcs Szarka, A. Kéry

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Gas and liquid chromatography coupled with mass spectrometry was applied to solve difficulties and reinvestigate the serious matrix problems affecting analysis of the active compounds in Euphrasia rostkoviana Hayne. The main groups of compounds were obtained by extracting the herb stepwise with n-hexane, chloroform, ethyl acetate and methanol. Polyamide column chromatography facilitated further separation. Phenolic/flavonoid- and terpenoid-type molecules were studied by GC-MS, HPLC and LC-MS-MS. The β-sitosterol content of the herb was determined by gas chromatography with flame ionisation detection (GC-FID). Caffeic acid, chlorogenic acid, coumaric acid and flavonoid glycosides of apigenin, luteolin, rhamnetine (hexoside), kaempferol (both hexoside and rutinoside) and quercetin (rutinoside) were identified in the fractions of the methanolic extract.

Original languageEnglish
JournalChromatographia
Volume68
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Euphrasia
Flavonoids
Gas chromatography
Gas Chromatography
Apigenin
Flame Ionization
Luteolin
Chlorogenic Acid
Coumaric Acids
Column chromatography
Nylons
Terpenes
Quercetin
Liquid chromatography
Glycosides
Chloroform
Ionization
Mass spectrometry
Methanol
Chromatography

Keywords

  • Column liquid chromatography-mass spectrometry
  • Euphrasia rostkoviana
  • Flavonoids
  • Gas chromatography-mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Investigation of Euphrasia rostkoviana Hayne using GC-MS and LC-MS. / Blazics, Balazs; Ludányi, K.; Szarka, Szabolcs; Kéry, A.

In: Chromatographia, Vol. 68, No. SUPPL. 1, 10.2008.

Research output: Contribution to journalArticle

Blazics, Balazs ; Ludányi, K. ; Szarka, Szabolcs ; Kéry, A. / Investigation of Euphrasia rostkoviana Hayne using GC-MS and LC-MS. In: Chromatographia. 2008 ; Vol. 68, No. SUPPL. 1.
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