Investigation of estrogen receptor α and β mRNA expression in the pregnant rat uterus

Renáta Minorics, Eszter Ducza, Árpád Márki, Eszter Páldy, George Falkay

Research output: Contribution to journalArticle

13 Citations (Scopus)


Although the expressions of the estrogen receptor (ER) subtypes have been demonstrated in a large number of estrogen target tissues, to date no evidence has been reported as to how the expressions of the alpha (α) and beta (β) ER subtype mRNA alter in the rat uterus during pregnancy. The aims of the present study were to obtain information concerning the changes in the ER and the progesterone receptor (PR) in early pregnancy and to determine the alterations in the ER subtype mRNA in the pregnant rat uterus. To demonstrate the ER and PR densities, radioligand saturation assay was used. The reverse-transcription-polymerase chain reaction (RT-PCR) was applied to characterize the alterations in the ER subtype mRNA. ER expression was highest on day 5 of pregnancy (Bmax=637.40±76.10 fmol/mg). The PR expression did not change significantly until day 8, but the protein density was increased on day 8 of pregnancy. The ERα mRNA expression was active during pregnancy, maximum expression was attained on day 5; a gradual decrease was then observed until the second half of pregnancy when its expression continuously increased up to the day of labor (day 22). Since the attachment of the blastocyst, an event that is dependent on estradiol in the progesterone-primed uterus of the rat, occurs on day 5 of pregnancy and the levels of the ER protein and the ERα mRNA on day 5 of pregnancy was the highest, it could be supposed that this subtype of ER might regulate implantation. The ERβ mRNA was detected only from day 7 to day 15, with a maximum level on day 8. The expression of this ER subtype might be related to the development of decidual tissue.

Original languageEnglish
Pages (from-to)463-468
Number of pages6
JournalMolecular Reproduction and Development
Issue number4
Publication statusPublished - Aug 1 2004


  • Decidua
  • Implantation
  • RT-PCR
  • Radioligand saturation assay

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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