Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction

Renáta Tóth, Vitor Cabral, Ernst Thuer, Flóra Bohner, Tibor Németh, Csaba Papp, Leonardo Nimrichter, Gergo Molnár, C. Vágvölgyi, Toni Gabaldón, Joshua D. Nosanchuk, A. Gácser

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Invasive candidiasis is among the most life-threatening infections in patients in intensive care units. Although Candida albicans is the leading cause of candidaemia, the incidence of Candida parapsilosis infections is also rising, particularly among the neonates. Due to differences in their biology, these species employ different antifungal resistance and virulence mechanisms and also induce dissimilar immune responses. Previously, it has been suggested that core virulence effecting transcription regulators could be attractive ligands for future antifungal drugs. Although the virulence regulatory mechanisms of C. albicans are well studied, less is known about similar mechanisms in C. parapsilosis. In order to search for potential targets for future antifungal drugs against this species, we analyzed the fungal transcriptome during host-pathogen interaction using an in vitro infection model. Selected genes with high expression levels were further examined through their respective null mutant strains, under conditions that mimic the host environment or influence pathogenicity. As a result, we identified several mutants with relevant pathogenicity affecting phenotypes. During the study we highlight three potentially tractable signaling regulators that influence C. parapsilosis pathogenicity in distinct mechanisms. During infection, CPAR2-100540 is responsible for nutrient acquisition, CPAR2-200390 for cell wall assembly and morphology switching and CPAR2-303700 for fungal viability.

Original languageEnglish
Article number1346
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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Host-Pathogen Interactions
Virulence Factors
Candida
Virulence
Infection
Candida albicans
Invasive Candidiasis
Candidemia
Transcriptome
Pharmaceutical Preparations
Cell Wall
Intensive Care Units
Newborn Infant
Ligands
Phenotype
Incidence
Genes

ASJC Scopus subject areas

  • General

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Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction. / Tóth, Renáta; Cabral, Vitor; Thuer, Ernst; Bohner, Flóra; Németh, Tibor; Papp, Csaba; Nimrichter, Leonardo; Molnár, Gergo; Vágvölgyi, C.; Gabaldón, Toni; Nosanchuk, Joshua D.; Gácser, A.

In: Scientific Reports, Vol. 8, No. 1, 1346, 01.12.2018.

Research output: Contribution to journalArticle

Tóth, R, Cabral, V, Thuer, E, Bohner, F, Németh, T, Papp, C, Nimrichter, L, Molnár, G, Vágvölgyi, C, Gabaldón, T, Nosanchuk, JD & Gácser, A 2018, 'Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction', Scientific Reports, vol. 8, no. 1, 1346. https://doi.org/10.1038/s41598-018-19453-4
Tóth, Renáta ; Cabral, Vitor ; Thuer, Ernst ; Bohner, Flóra ; Németh, Tibor ; Papp, Csaba ; Nimrichter, Leonardo ; Molnár, Gergo ; Vágvölgyi, C. ; Gabaldón, Toni ; Nosanchuk, Joshua D. ; Gácser, A. / Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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