Investigation of Archaeal and Bacterial community structure of five different small drinking water networks with special regard to the nitrifying microorganisms

Zsuzsanna Nagymáté, Zalán G. Homonnay, K. Márialigeti

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Total microbial community structure, and particularly nitrifying communities inhabiting five different small drinking water networks characterized with different water physical and chemical parameters was investigated, using cultivation-based methods and sequence aided Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis. Ammonium ion, originated from well water, was only partially oxidized via nitrite to nitrate in the drinking water distribution systems. Nitrification occurred at low ammonium ion concentration (27-46 μM), relatively high pH (7.6-8.2) and over a wide range of dissolved oxygen concentrations (0.4-9.0 mg L-1). The nitrifying communities of the distribution systems were characterized by variable most probable numbers (2 × 102-7.1 × 104 MPN L-1) and probably originated from the non-treated well water. The sequence aided T-RFLP method revealed that ammonia-oxidizing microorganisms and nitrite-oxidizing Bacteria (Nitrosomonas oligotropha, Nitrosopumilus maritimus, and Nitrospira moscoviensis, 'Candidatus Nitrospira defluvii') were present in different ratios in the total microbial communities of the distinct parts of the water network systems. The nitrate generated by nitrification was partly utilized by nitrate-reducing (and denitrifying) Bacteria, present in low MPN and characterized by sequence aided T-RFLP as Comamonas sp. and Pseudomonas spp. Different environmental factors, like pH, chemical oxygen demand, calculated total inorganic nitrogen content (moreover nitrite and nitrate concentration), temperature had important effect on the total bacterial and archaeal community distribution.

Original languageEnglish
Pages (from-to)80-89
Number of pages10
JournalMicrobiological Research
Volume188-189
DOIs
Publication statusPublished - Jul 1 2016

Fingerprint

Bacterial Structures
Drinking Water
Nitrates
Nitrites
Restriction Fragment Length Polymorphisms
Nitrification
Water
Ammonium Compounds
Comamonas
Nitrosomonas
Ions
Bacteria
Biological Oxygen Demand Analysis
Pseudomonas
Ammonia
Nitrogen
Oxygen
Temperature

Keywords

  • Ammonia-oxidizing archaea
  • Ammonia-oxidizing bacteria
  • AmoA
  • Drinking water
  • Most probable number
  • Sequence aided T-RFLP

ASJC Scopus subject areas

  • Microbiology

Cite this

Investigation of Archaeal and Bacterial community structure of five different small drinking water networks with special regard to the nitrifying microorganisms. / Nagymáté, Zsuzsanna; Homonnay, Zalán G.; Márialigeti, K.

In: Microbiological Research, Vol. 188-189, 01.07.2016, p. 80-89.

Research output: Contribution to journalArticle

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