Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction

Dinko Novosel, Daniel Cadar, T. Tuboly, Andreja Jungic, Tomasz Stadejek, Tahar Ait-Ali, Attila Cságola

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Porcine parvovirus 2 (PPV2) was detected in swine serum without showing any relationship with disease. The emergence of the virus seemed to be a unique event until other genetically highly similar parvoviruses were identified in China and, later in 2012, the presence of the virus was also described in Europe. PPV2 is widely distributed in pig populations where it is suspected to be involved in respiratory conditions, based on its frequent detection in lung samples. In order to investigate the potential pathogenic involvement of PPV2, 60 dead pigs were examined from two farms. They were necropsied and tested for PPV2 and PCV2 (Porcine circovirus type 2) by PCR; by Brown and Brenn (B&B) staining for bacteria; by immunohistochemistry (IHC) to detect CD3, Swine leukocyte antigen class II DQ (SLAIIDQ), lysozyme, porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza (SIV), Mycoplasma hyopneumoniae (Mhyo); and by in situ hybridization (ISH) to detect ssDNA and dsDNA of PCV2. PPV2 positive samples were subjected to in situ polymerase chain reaction (IS-PCR) including double staining method to detect PPV2 and host cell markers. To calculate statistical difference we used GENMOD or LOGISTIC procedures in Statistical Analysis System (SAS®). Results: We found that the PPV2 was localized mostly in lymphocytes in lungs, lymph nodes and liver. Neither CD3 antigen nor lysozyme was expressed by these infected cells. In contrast, low levels of SLAIIDQ were expressed by infected cells, suggesting that PPV2 may have a specific tropism for immature B lymphocytes and/or NK lymphocytes though possibly not T lymphocytes. Conclusion: The overall conclusion of this study indicates that PPV2 may contribute to the pathogenesis of pneumonia.

Original languageEnglish
Article number163
JournalBMC Veterinary Research
Volume14
Issue number1
DOIs
Publication statusPublished - May 21 2018

Fingerprint

Porcine Parvovirus
Ungulate protoparvovirus 1
polymerase chain reaction
Genotype
Polymerase Chain Reaction
Infection
infection
Swine
swine
Circovirus
Porcine circovirus-2
Muramidase
antigens
lysozyme
leukocytes
Parvoviridae
lymphocytes
Mycoplasma hyopneumoniae
lungs
Lymphocytes

Keywords

  • In situ PCR
  • Lungs
  • Lymphocytes
  • PPV2

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction. / Novosel, Dinko; Cadar, Daniel; Tuboly, T.; Jungic, Andreja; Stadejek, Tomasz; Ait-Ali, Tahar; Cságola, Attila.

In: BMC Veterinary Research, Vol. 14, No. 1, 163, 21.05.2018.

Research output: Contribution to journalArticle

Novosel, Dinko ; Cadar, Daniel ; Tuboly, T. ; Jungic, Andreja ; Stadejek, Tomasz ; Ait-Ali, Tahar ; Cságola, Attila. / Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction. In: BMC Veterinary Research. 2018 ; Vol. 14, No. 1.
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abstract = "Background: Porcine parvovirus 2 (PPV2) was detected in swine serum without showing any relationship with disease. The emergence of the virus seemed to be a unique event until other genetically highly similar parvoviruses were identified in China and, later in 2012, the presence of the virus was also described in Europe. PPV2 is widely distributed in pig populations where it is suspected to be involved in respiratory conditions, based on its frequent detection in lung samples. In order to investigate the potential pathogenic involvement of PPV2, 60 dead pigs were examined from two farms. They were necropsied and tested for PPV2 and PCV2 (Porcine circovirus type 2) by PCR; by Brown and Brenn (B&B) staining for bacteria; by immunohistochemistry (IHC) to detect CD3, Swine leukocyte antigen class II DQ (SLAIIDQ), lysozyme, porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza (SIV), Mycoplasma hyopneumoniae (Mhyo); and by in situ hybridization (ISH) to detect ssDNA and dsDNA of PCV2. PPV2 positive samples were subjected to in situ polymerase chain reaction (IS-PCR) including double staining method to detect PPV2 and host cell markers. To calculate statistical difference we used GENMOD or LOGISTIC procedures in Statistical Analysis System (SAS{\circledR}). Results: We found that the PPV2 was localized mostly in lymphocytes in lungs, lymph nodes and liver. Neither CD3 antigen nor lysozyme was expressed by these infected cells. In contrast, low levels of SLAIIDQ were expressed by infected cells, suggesting that PPV2 may have a specific tropism for immature B lymphocytes and/or NK lymphocytes though possibly not T lymphocytes. Conclusion: The overall conclusion of this study indicates that PPV2 may contribute to the pathogenesis of pneumonia.",
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