Inverse hierarchy of vimentin epitope expression in primary cultures of chicken and rat astrocytes: A double-immunofluorescence study

B. Gereben, K. Leuhuber, W. D. Rausch, P. Galfi, V. Jancsik, P. Rudas

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Vimentin contributes to the cytoskeleton of different cell-types, among them glial cells. We report here that different forms of this protein, distinguishable by the monoclonal antibodies Vim3B4 and V9, are species- specifically expressed in cultures of glial fibrillary acidic protein (GFAP) positive, primary astrocytes of the chicken and rat. Most cells in the cultures co-expressed GFAP and one of the two vimentin epitopes. The Vim3B4 positive epitope was present in chicken astrocytes, while the V9 positive was not. Inverse situation was found in the astrocytes of rat. In vitro age of the cells did not influence the hierarchy of vimentin epitope expression with respect to species-specificity. Our result shows that the different vimentin expression program of cultured astrocytes of the chicken and rat is preserved under in vitro conditions. The presented data support the concept of the species-specific regulation of vimentin forms in glial cells of the central nervous system.

Original languageEnglish
Pages (from-to)141-150
Number of pages10
JournalNeurobiology
Volume6
Issue number2
Publication statusPublished - 1998

Fingerprint

Vimentin
Astrocytes
Fluorescent Antibody Technique
Epitopes
Chickens
Glial Fibrillary Acidic Protein
Neuroglia
Species Specificity
Cytoskeleton
Central Nervous System
Cell Culture Techniques
Monoclonal Antibodies
Proteins
In Vitro Techniques

Keywords

  • Astrocytes
  • Cell-culture
  • Chicken
  • Rat
  • Vimentin epitopes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inverse hierarchy of vimentin epitope expression in primary cultures of chicken and rat astrocytes : A double-immunofluorescence study. / Gereben, B.; Leuhuber, K.; Rausch, W. D.; Galfi, P.; Jancsik, V.; Rudas, P.

In: Neurobiology, Vol. 6, No. 2, 1998, p. 141-150.

Research output: Contribution to journalArticle

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AU - Jancsik, V.

AU - Rudas, P.

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