Introduction of a method for three-dimensional mapping of the charge motion in bacteriorhodopsin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Electric signals associated with the photocycle of bacteriorhodopsin carry valuable information about the proton transport process. Photocurrents measured by different experimental methods are interpreted in terms of intramolecular charge displacements [1,2]. Permanent electrical asymmetry of the sample is considered to be a prerequisite for the detection of electric signals. The various photoelectric measuring techniques can be distinguished by the way of achievement of this asymmetry [3]. A common feature of the available methods, however, is that the samples are cylindrically symmetric. Consequently, intramembraneous charge displacements can normally be monitored only along the axis of the membrane normal. We developed a novel method that allows also the detection of the in-plane components of the charge displacements. Samples containing oriented purple membrane fragments were used in the experiments [4], and the rotational symmetry was transiently broken via anisotropic excitation of the bR molecules by linearly polarized light. Kinetics of the normal and in-plane components were measured and interpreted as a result of spatial charge displacements associated with the proton transport process in bacteriorhodopsin.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalBiophysical Chemistry
Volume56
Issue number1-2
DOIs
Publication statusPublished - 1995

Fingerprint

Bacteriorhodopsins
Protons
Membranes
Light polarization
Photocurrents
Purple Membrane
asymmetry
membranes
Molecules
Kinetics
protons
polarized light
photocurrents
Light
Experiments
fragments
kinetics
symmetry
excitation
molecules

Keywords

  • Charge displacements
  • Orientation
  • Photoselection
  • Purple membrane

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Organic Chemistry
  • Physical and Theoretical Chemistry

Cite this

Introduction of a method for three-dimensional mapping of the charge motion in bacteriorhodopsin. / Dér, A.; Ormos, P.

In: Biophysical Chemistry, Vol. 56, No. 1-2, 1995, p. 159-163.

Research output: Contribution to journalArticle

@article{75e92f25937d4777ac2f93bf1f2718a9,
title = "Introduction of a method for three-dimensional mapping of the charge motion in bacteriorhodopsin",
abstract = "Electric signals associated with the photocycle of bacteriorhodopsin carry valuable information about the proton transport process. Photocurrents measured by different experimental methods are interpreted in terms of intramolecular charge displacements [1,2]. Permanent electrical asymmetry of the sample is considered to be a prerequisite for the detection of electric signals. The various photoelectric measuring techniques can be distinguished by the way of achievement of this asymmetry [3]. A common feature of the available methods, however, is that the samples are cylindrically symmetric. Consequently, intramembraneous charge displacements can normally be monitored only along the axis of the membrane normal. We developed a novel method that allows also the detection of the in-plane components of the charge displacements. Samples containing oriented purple membrane fragments were used in the experiments [4], and the rotational symmetry was transiently broken via anisotropic excitation of the bR molecules by linearly polarized light. Kinetics of the normal and in-plane components were measured and interpreted as a result of spatial charge displacements associated with the proton transport process in bacteriorhodopsin.",
keywords = "Charge displacements, Orientation, Photoselection, Purple membrane",
author = "A. D{\'e}r and P. Ormos",
year = "1995",
doi = "10.1016/0301-4622(95)00028-V",
language = "English",
volume = "56",
pages = "159--163",
journal = "Biophysical Chemistry",
issn = "0301-4622",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Introduction of a method for three-dimensional mapping of the charge motion in bacteriorhodopsin

AU - Dér, A.

AU - Ormos, P.

PY - 1995

Y1 - 1995

N2 - Electric signals associated with the photocycle of bacteriorhodopsin carry valuable information about the proton transport process. Photocurrents measured by different experimental methods are interpreted in terms of intramolecular charge displacements [1,2]. Permanent electrical asymmetry of the sample is considered to be a prerequisite for the detection of electric signals. The various photoelectric measuring techniques can be distinguished by the way of achievement of this asymmetry [3]. A common feature of the available methods, however, is that the samples are cylindrically symmetric. Consequently, intramembraneous charge displacements can normally be monitored only along the axis of the membrane normal. We developed a novel method that allows also the detection of the in-plane components of the charge displacements. Samples containing oriented purple membrane fragments were used in the experiments [4], and the rotational symmetry was transiently broken via anisotropic excitation of the bR molecules by linearly polarized light. Kinetics of the normal and in-plane components were measured and interpreted as a result of spatial charge displacements associated with the proton transport process in bacteriorhodopsin.

AB - Electric signals associated with the photocycle of bacteriorhodopsin carry valuable information about the proton transport process. Photocurrents measured by different experimental methods are interpreted in terms of intramolecular charge displacements [1,2]. Permanent electrical asymmetry of the sample is considered to be a prerequisite for the detection of electric signals. The various photoelectric measuring techniques can be distinguished by the way of achievement of this asymmetry [3]. A common feature of the available methods, however, is that the samples are cylindrically symmetric. Consequently, intramembraneous charge displacements can normally be monitored only along the axis of the membrane normal. We developed a novel method that allows also the detection of the in-plane components of the charge displacements. Samples containing oriented purple membrane fragments were used in the experiments [4], and the rotational symmetry was transiently broken via anisotropic excitation of the bR molecules by linearly polarized light. Kinetics of the normal and in-plane components were measured and interpreted as a result of spatial charge displacements associated with the proton transport process in bacteriorhodopsin.

KW - Charge displacements

KW - Orientation

KW - Photoselection

KW - Purple membrane

UR - http://www.scopus.com/inward/record.url?scp=0029123221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029123221&partnerID=8YFLogxK

U2 - 10.1016/0301-4622(95)00028-V

DO - 10.1016/0301-4622(95)00028-V

M3 - Article

AN - SCOPUS:0029123221

VL - 56

SP - 159

EP - 163

JO - Biophysical Chemistry

JF - Biophysical Chemistry

SN - 0301-4622

IS - 1-2

ER -