The role of corticotrophin-releasing factor (CRF) and prostaglandin in interleukin-1Β (IL-1Β)-induced delayed gastric emptying was investigated. Gastric emptying was monitored 20 min after orogastric delivery of a methylcellulose phenol red solution in fasted rats injected intravenously with IL-1Β at the ED50 dose (3 ng/rat) 30 min before the non-caloric solution. The IL-1 receptor antagonist (IL-1ra) injected intravenously (3 μg/rat) or intracisternally (100 ng/rat) reversed the IL-1Β-induced 47% inhibition of gastric emptying by 100% and 62%, respectively. The new CRF antagonist [DPhe12, Nle21 > 38, CαMeLeu37]-CRF12-41 (20 μg/rat), injected intracisternally, or indomethacin (5 mg/kg i.p.) completely abolished IL-1Β (3 ng/rat i.v.)-induced gastric stasis. The CRF antagonist injected intravenously (20 μg/rat) did not influence the IL-1Β inhibitory action. None of the pretreatments given alone influenced basal gastric emptying. These data suggest that peripheral IL-1Β-induced inhibition of gastric emptying is mediated by specific IL-1 receptor interactions and brain CRF pathways requiring the integrity of eicosanoid-cyclooxygenase pathways.
- Corticotrophin-releasing factor
- Gastric emptying
- Interleukin-receptor antagonist
ASJC Scopus subject areas