Abstract
The role of corticotrophin-releasing factor (CRF) and prostaglandin in interleukin-1β (IL-1β)-induced delayed gastric emptying was investigated. Gastric emptying was monitored 20 min after orogastric delivery of a methylcellulose phenol red solution in fasted rats injected intravenously with IL-1β at the ED50 dose (3 ng/rat) 30 min before the non-caloric solution. The IL-1 receptor antagonist (IL-1ra) injected intravenously (3 μg/rat) or intracisternally (100 ng/rat) reversed the IL-1β-induced 47% inhibition of gastric emptying by 100% and 62%, respectively. The new CRF antagonist [DPhe12, Nle21,38, C(α)MeLeu37]-CRF12-41 (20 μg/rat), injected intracisternally, or indomethacin (5 mg/kg i.p.) completely abolished IL-1β (3 ng/rat i.v.)-induced gastric stasis. The CRF antagonist injected intravenously (20 μg/rat) did not influence the IL-1β inhibitory action. None of the pretreatments given alone influenced basal gastric emptying. These data suggest that peripheral IL-1β-induced inhibition of gastric emptying is mediated by specific IL-1 receptor interactions and brain CRF pathways requiring the integrity of eicosanoid-cyclooxygenase pathways.
| Original language | English |
|---|---|
| Pages (from-to) | 135-140 |
| Number of pages | 6 |
| Journal | Digestion |
| Volume | 57 |
| Issue number | 2 |
| Publication status | Published - Mar 1996 |
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Keywords
- Antagonist, CRF
- Corticotrophin-releasing factor
- Gastric emptying
- Interleukin-1-beta
- Interleukin-receptor antagonist
- Prostaglandin
ASJC Scopus subject areas
- Gastroenterology
Cite this
Intravenous interleukin-1-beta-induced inhibition of gastric emptying : Involvement of central corticotrophin-releasing factor and prostaglandin pathways in rats. / Sütö, G.; Kiraly, Á; Plourde, V.; Taché, Y.
In: Digestion, Vol. 57, No. 2, 03.1996, p. 135-140.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intravenous interleukin-1-beta-induced inhibition of gastric emptying
T2 - Involvement of central corticotrophin-releasing factor and prostaglandin pathways in rats
AU - Sütö, G.
AU - Kiraly, Á
AU - Plourde, V.
AU - Taché, Y.
PY - 1996/3
Y1 - 1996/3
N2 - The role of corticotrophin-releasing factor (CRF) and prostaglandin in interleukin-1β (IL-1β)-induced delayed gastric emptying was investigated. Gastric emptying was monitored 20 min after orogastric delivery of a methylcellulose phenol red solution in fasted rats injected intravenously with IL-1β at the ED50 dose (3 ng/rat) 30 min before the non-caloric solution. The IL-1 receptor antagonist (IL-1ra) injected intravenously (3 μg/rat) or intracisternally (100 ng/rat) reversed the IL-1β-induced 47% inhibition of gastric emptying by 100% and 62%, respectively. The new CRF antagonist [DPhe12, Nle21,38, C(α)MeLeu37]-CRF12-41 (20 μg/rat), injected intracisternally, or indomethacin (5 mg/kg i.p.) completely abolished IL-1β (3 ng/rat i.v.)-induced gastric stasis. The CRF antagonist injected intravenously (20 μg/rat) did not influence the IL-1β inhibitory action. None of the pretreatments given alone influenced basal gastric emptying. These data suggest that peripheral IL-1β-induced inhibition of gastric emptying is mediated by specific IL-1 receptor interactions and brain CRF pathways requiring the integrity of eicosanoid-cyclooxygenase pathways.
AB - The role of corticotrophin-releasing factor (CRF) and prostaglandin in interleukin-1β (IL-1β)-induced delayed gastric emptying was investigated. Gastric emptying was monitored 20 min after orogastric delivery of a methylcellulose phenol red solution in fasted rats injected intravenously with IL-1β at the ED50 dose (3 ng/rat) 30 min before the non-caloric solution. The IL-1 receptor antagonist (IL-1ra) injected intravenously (3 μg/rat) or intracisternally (100 ng/rat) reversed the IL-1β-induced 47% inhibition of gastric emptying by 100% and 62%, respectively. The new CRF antagonist [DPhe12, Nle21,38, C(α)MeLeu37]-CRF12-41 (20 μg/rat), injected intracisternally, or indomethacin (5 mg/kg i.p.) completely abolished IL-1β (3 ng/rat i.v.)-induced gastric stasis. The CRF antagonist injected intravenously (20 μg/rat) did not influence the IL-1β inhibitory action. None of the pretreatments given alone influenced basal gastric emptying. These data suggest that peripheral IL-1β-induced inhibition of gastric emptying is mediated by specific IL-1 receptor interactions and brain CRF pathways requiring the integrity of eicosanoid-cyclooxygenase pathways.
KW - Antagonist, CRF
KW - Corticotrophin-releasing factor
KW - Gastric emptying
KW - Interleukin-1-beta
KW - Interleukin-receptor antagonist
KW - Prostaglandin
UR - http://www.scopus.com/inward/record.url?scp=0029936861&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029936861&partnerID=8YFLogxK
M3 - Article
VL - 57
SP - 135
EP - 140
JO - Digestion
JF - Digestion
SN - 0012-2823
IS - 2
ER -