Intravenous interleukin-1-beta-induced inhibition of gastric emptying: Involvement of central corticotrophin-releasing factor and prostaglandin pathways in rats

G. Sütö, Á Kiraly, V. Plourde, Y. Taché

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The role of corticotrophin-releasing factor (CRF) and prostaglandin in interleukin-1β (IL-1β)-induced delayed gastric emptying was investigated. Gastric emptying was monitored 20 min after orogastric delivery of a methylcellulose phenol red solution in fasted rats injected intravenously with IL-1β at the ED50 dose (3 ng/rat) 30 min before the non-caloric solution. The IL-1 receptor antagonist (IL-1ra) injected intravenously (3 μg/rat) or intracisternally (100 ng/rat) reversed the IL-1β-induced 47% inhibition of gastric emptying by 100% and 62%, respectively. The new CRF antagonist [DPhe12, Nle21,38, C(α)MeLeu37]-CRF12-41 (20 μg/rat), injected intracisternally, or indomethacin (5 mg/kg i.p.) completely abolished IL-1β (3 ng/rat i.v.)-induced gastric stasis. The CRF antagonist injected intravenously (20 μg/rat) did not influence the IL-1β inhibitory action. None of the pretreatments given alone influenced basal gastric emptying. These data suggest that peripheral IL-1β-induced inhibition of gastric emptying is mediated by specific IL-1 receptor interactions and brain CRF pathways requiring the integrity of eicosanoid-cyclooxygenase pathways.

Original languageEnglish
Pages (from-to)135-140
Number of pages6
JournalDigestion
Volume57
Issue number2
Publication statusPublished - Mar 1996

Fingerprint

Gastric Emptying
Corticotropin-Releasing Hormone
Interleukin-1beta
Prostaglandins
Interleukin-1
Interleukin-1 Receptors
Phenolsulfonphthalein
Gastroparesis
Methylcellulose
Eicosanoids
Interleukin-3
Prostaglandin-Endoperoxide Synthases
Indomethacin
Brain

Keywords

  • Antagonist, CRF
  • Corticotrophin-releasing factor
  • Gastric emptying
  • Interleukin-1-beta
  • Interleukin-receptor antagonist
  • Prostaglandin

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Intravenous interleukin-1-beta-induced inhibition of gastric emptying : Involvement of central corticotrophin-releasing factor and prostaglandin pathways in rats. / Sütö, G.; Kiraly, Á; Plourde, V.; Taché, Y.

In: Digestion, Vol. 57, No. 2, 03.1996, p. 135-140.

Research output: Contribution to journalArticle

@article{073d32c2075244de9d7f4c280ab60f7e,
title = "Intravenous interleukin-1-beta-induced inhibition of gastric emptying: Involvement of central corticotrophin-releasing factor and prostaglandin pathways in rats",
abstract = "The role of corticotrophin-releasing factor (CRF) and prostaglandin in interleukin-1β (IL-1β)-induced delayed gastric emptying was investigated. Gastric emptying was monitored 20 min after orogastric delivery of a methylcellulose phenol red solution in fasted rats injected intravenously with IL-1β at the ED50 dose (3 ng/rat) 30 min before the non-caloric solution. The IL-1 receptor antagonist (IL-1ra) injected intravenously (3 μg/rat) or intracisternally (100 ng/rat) reversed the IL-1β-induced 47{\%} inhibition of gastric emptying by 100{\%} and 62{\%}, respectively. The new CRF antagonist [DPhe12, Nle21,38, C(α)MeLeu37]-CRF12-41 (20 μg/rat), injected intracisternally, or indomethacin (5 mg/kg i.p.) completely abolished IL-1β (3 ng/rat i.v.)-induced gastric stasis. The CRF antagonist injected intravenously (20 μg/rat) did not influence the IL-1β inhibitory action. None of the pretreatments given alone influenced basal gastric emptying. These data suggest that peripheral IL-1β-induced inhibition of gastric emptying is mediated by specific IL-1 receptor interactions and brain CRF pathways requiring the integrity of eicosanoid-cyclooxygenase pathways.",
keywords = "Antagonist, CRF, Corticotrophin-releasing factor, Gastric emptying, Interleukin-1-beta, Interleukin-receptor antagonist, Prostaglandin",
author = "G. S{\"u}t{\"o} and {\'A} Kiraly and V. Plourde and Y. Tach{\'e}",
year = "1996",
month = "3",
language = "English",
volume = "57",
pages = "135--140",
journal = "Digestion",
issn = "0012-2823",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Intravenous interleukin-1-beta-induced inhibition of gastric emptying

T2 - Involvement of central corticotrophin-releasing factor and prostaglandin pathways in rats

AU - Sütö, G.

AU - Kiraly, Á

AU - Plourde, V.

AU - Taché, Y.

PY - 1996/3

Y1 - 1996/3

N2 - The role of corticotrophin-releasing factor (CRF) and prostaglandin in interleukin-1β (IL-1β)-induced delayed gastric emptying was investigated. Gastric emptying was monitored 20 min after orogastric delivery of a methylcellulose phenol red solution in fasted rats injected intravenously with IL-1β at the ED50 dose (3 ng/rat) 30 min before the non-caloric solution. The IL-1 receptor antagonist (IL-1ra) injected intravenously (3 μg/rat) or intracisternally (100 ng/rat) reversed the IL-1β-induced 47% inhibition of gastric emptying by 100% and 62%, respectively. The new CRF antagonist [DPhe12, Nle21,38, C(α)MeLeu37]-CRF12-41 (20 μg/rat), injected intracisternally, or indomethacin (5 mg/kg i.p.) completely abolished IL-1β (3 ng/rat i.v.)-induced gastric stasis. The CRF antagonist injected intravenously (20 μg/rat) did not influence the IL-1β inhibitory action. None of the pretreatments given alone influenced basal gastric emptying. These data suggest that peripheral IL-1β-induced inhibition of gastric emptying is mediated by specific IL-1 receptor interactions and brain CRF pathways requiring the integrity of eicosanoid-cyclooxygenase pathways.

AB - The role of corticotrophin-releasing factor (CRF) and prostaglandin in interleukin-1β (IL-1β)-induced delayed gastric emptying was investigated. Gastric emptying was monitored 20 min after orogastric delivery of a methylcellulose phenol red solution in fasted rats injected intravenously with IL-1β at the ED50 dose (3 ng/rat) 30 min before the non-caloric solution. The IL-1 receptor antagonist (IL-1ra) injected intravenously (3 μg/rat) or intracisternally (100 ng/rat) reversed the IL-1β-induced 47% inhibition of gastric emptying by 100% and 62%, respectively. The new CRF antagonist [DPhe12, Nle21,38, C(α)MeLeu37]-CRF12-41 (20 μg/rat), injected intracisternally, or indomethacin (5 mg/kg i.p.) completely abolished IL-1β (3 ng/rat i.v.)-induced gastric stasis. The CRF antagonist injected intravenously (20 μg/rat) did not influence the IL-1β inhibitory action. None of the pretreatments given alone influenced basal gastric emptying. These data suggest that peripheral IL-1β-induced inhibition of gastric emptying is mediated by specific IL-1 receptor interactions and brain CRF pathways requiring the integrity of eicosanoid-cyclooxygenase pathways.

KW - Antagonist, CRF

KW - Corticotrophin-releasing factor

KW - Gastric emptying

KW - Interleukin-1-beta

KW - Interleukin-receptor antagonist

KW - Prostaglandin

UR - http://www.scopus.com/inward/record.url?scp=0029936861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029936861&partnerID=8YFLogxK

M3 - Article

C2 - 8786002

AN - SCOPUS:0029936861

VL - 57

SP - 135

EP - 140

JO - Digestion

JF - Digestion

SN - 0012-2823

IS - 2

ER -