Intrastrain and interstrain variability in biotransformation enzyme activities of rainbow trout (Oncorhynchus mykiss)

Kari Koponen, Ossi Ritola, Sirpa Huuskonen, Dan Linder, K. Monostory, Pirjo Lindström-Seppä

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We assessed the intrastrain and interstrain variability of cytochrome P450 enzyme activities and conjugation reactions of different rainbow trout (Oncorhynchus mykiss) strains. The study protocol was divided into two parts: in two separate experiments, monooxygenase (7-ethoxyresorufin O-deethylase, EROD) and conjugation (glutathione S-transferase, GST; UDP-glucuronosyl transferase, UDPGT) enzyme activities were measured from liver and kidney samples of commonly used rainbow trout breeds. In the preliminary study, the differences in the basal biotransformation enzyme activities were detected in two separately cultivated rainbow trout strains. Results indicated significant intrastrain (UDPGT, GST) and interstrain (EROD) differences in biotransformation enzyme activities. In the experimental study, corresponding enzyme activities were measured from three different rainbow trout strains exposed to β-naphthoflavone (BNF), a polycyclic aromatic hydrocarbon (PAH) type model compound. The results revealed significant interstrain differences, especially in hepatic EROD and GST activities. However, UDPGT activities in the liver, as well as the measured monooxygenase and conjugation activities in the kidney, showed no notable variance between strains. The present work addresses intrastrain differences between hatcheries and highlights the need for intercalibration of rainbow trout strains used in experimental studies in aquatic toxicology.

Original languageEnglish
Pages (from-to)2901-2906
Number of pages6
JournalCanadian Journal of Fisheries and Aquatic Sciences
Volume54
Issue number12
DOIs
Publication statusPublished - 1997

Fingerprint

biotransformation
rainbow
enzyme activity
Oncorhynchus mykiss
experimental study
liver
kidneys
toxicology
hatchery
cytochrome
polycyclic aromatic hydrocarbons
transferases
PAH
glutathione transferase
cytochrome P-450
hatcheries
breeds
experiment

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Intrastrain and interstrain variability in biotransformation enzyme activities of rainbow trout (Oncorhynchus mykiss). / Koponen, Kari; Ritola, Ossi; Huuskonen, Sirpa; Linder, Dan; Monostory, K.; Lindström-Seppä, Pirjo.

In: Canadian Journal of Fisheries and Aquatic Sciences, Vol. 54, No. 12, 1997, p. 2901-2906.

Research output: Contribution to journalArticle

Koponen, Kari ; Ritola, Ossi ; Huuskonen, Sirpa ; Linder, Dan ; Monostory, K. ; Lindström-Seppä, Pirjo. / Intrastrain and interstrain variability in biotransformation enzyme activities of rainbow trout (Oncorhynchus mykiss). In: Canadian Journal of Fisheries and Aquatic Sciences. 1997 ; Vol. 54, No. 12. pp. 2901-2906.
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