Intrarenal generation of angiotensin II evaluated by an electrophysiological technique

C. P. Buhrle, L. Rosivall, R. Taugner

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

Angiotensin II (ANG II) reversibly depolarizes renin-containing juxtaglomerular epithelioid cells (JGECs) of the hydronephrotic mouse kidney afferent arteriole. This depolarizing response was utilized to assess changes in ANG II concentration in the vicinity of JGECs in order to test whether ANG II is generated from ANG I and artificial renin substrate (ARS) in this preparation. Depolarizations were also produced by the application of ANG I and ARS in the superfusing medium. These responses to ANG I and ARS were completely blocked by saralasin. Hence, our findings are indicative for an intrarenal, local generation of ANG II. As opposed to saralasin, several converting enzyme and renin inhibitors only diminished but generally did not abolish the actions of ANG I and ARS, respectively. These results suggest an alternative, nonrenin and non-converting enzyme-dependent pathway of ANG II generation in renal tissue.

Original languageEnglish
Title of host publicationAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume252
Edition4
Publication statusPublished - 1987

Fingerprint

Angiotensinogen
Angiotensin II
Saralasin
Epithelioid Cells
Renin
Kidney
Arterioles
Enzyme Inhibitors
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Buhrle, C. P., Rosivall, L., & Taugner, R. (1987). Intrarenal generation of angiotensin II evaluated by an electrophysiological technique. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology (4 ed., Vol. 252)

Intrarenal generation of angiotensin II evaluated by an electrophysiological technique. / Buhrle, C. P.; Rosivall, L.; Taugner, R.

American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 252 4. ed. 1987.

Research output: Chapter in Book/Report/Conference proceedingChapter

Buhrle, CP, Rosivall, L & Taugner, R 1987, Intrarenal generation of angiotensin II evaluated by an electrophysiological technique. in American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 4 edn, vol. 252.
Buhrle CP, Rosivall L, Taugner R. Intrarenal generation of angiotensin II evaluated by an electrophysiological technique. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 4 ed. Vol. 252. 1987
Buhrle, C. P. ; Rosivall, L. ; Taugner, R. / Intrarenal generation of angiotensin II evaluated by an electrophysiological technique. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 252 4. ed. 1987.
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