Intrarenal angiotensin I conversion at normal and reduced renal blood flow in the dog.

L. Rosivall, D. F. Rinder, J. Champion, M. C. Khosla, L. G. Navar, S. Oparil

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Intrarenal conversion of angiotensin I (ANG I) to angiotensin II (ANG II) under conditions of normal and reduced renal blood flow (RBF) elicited by constriction of the renal artery was examined in pentobarbital-anesthetized dogs. In eight animals, tracer doses of 125I-ANG I (5-12 pmol) were injected into the renal artery and 125I-ANG I, 125I-ANG II, and 125I-labeled metabolites were measured in renal venous effluent by high-voltage paper electrophoresis. The mean conversion of ANG I to ANG II during a single passage through the kidney was 21.8 +/- 2.1% at control RBF. When RBF was decreased by 25 and 53%, percent ANG I conversion was not altered significantly. In six dogs percent conversion of 125I-[Sar1, Ile5]ANG I, an ANG I analogue refractory to hydrolysis by aminopeptidases, was 18.1 +/- 1.7% at control RBF and did not change significantly when the RBF was reduced by 55%. Although there were severalfold increases in renal renin secretion rate and net ANG I generation rate during reduced RBF, net renal ANG II formation rate did not change significantly. These data indicate that there is substantial conversion of ANG I in a single passage through the dog kidney and that intrarenal ANG I conversion is independent of RBF even under conditions in which renin secretion rate and ANG I generation rate are increased severalfold.

Original languageEnglish
JournalThe American journal of physiology
Volume245
Issue number3
Publication statusPublished - Sep 1983

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Angiotensin I
Renal Circulation
Dogs
Angiotensin II
Kidney
Renal Artery
Renin
Paper Electrophoresis
Aminopeptidases
Pentobarbital
Constriction
Hydrolysis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Rosivall, L., Rinder, D. F., Champion, J., Khosla, M. C., Navar, L. G., & Oparil, S. (1983). Intrarenal angiotensin I conversion at normal and reduced renal blood flow in the dog. The American journal of physiology, 245(3).

Intrarenal angiotensin I conversion at normal and reduced renal blood flow in the dog. / Rosivall, L.; Rinder, D. F.; Champion, J.; Khosla, M. C.; Navar, L. G.; Oparil, S.

In: The American journal of physiology, Vol. 245, No. 3, 09.1983.

Research output: Contribution to journalArticle

Rosivall, L, Rinder, DF, Champion, J, Khosla, MC, Navar, LG & Oparil, S 1983, 'Intrarenal angiotensin I conversion at normal and reduced renal blood flow in the dog.', The American journal of physiology, vol. 245, no. 3.
Rosivall, L. ; Rinder, D. F. ; Champion, J. ; Khosla, M. C. ; Navar, L. G. ; Oparil, S. / Intrarenal angiotensin I conversion at normal and reduced renal blood flow in the dog. In: The American journal of physiology. 1983 ; Vol. 245, No. 3.
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