Intraovarian transplantation of stage I-II follicles results in viable zebrafish embryos

Zsolt Csenki, Andreas Zaucker, Balázs Kovács, Yavor Hadzhiev, Árpád Hegyi, Katalin Kinga Lefler, Tamás Müller, Róbert Kovács, B. Urbányi, László Váradi, Ferenc Müller

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Maternal gene products drive early embryogenesis almost exclusively until the mid blastula transition (MBT) in many animal models including fish. However, the maternal contribution to embryogenesis does not stop at MBT, but continues to be an essential regulator of key developmental processes. The extent to which maternal effects contribute to embryonic and larval development is hard to estimate due to the technical difficulty of interfering with maternal gene products by conventional forward and reverse genetic tools. Therefore, novel methods to manipulate maternal factors in oocytes need to be developed. Here, we provide a proof of principle protocol for transplanting stage I-II zebrafish follicles into recipient mothers where donor stage I oocytes can develop to stage IV in 2 weeks and in 3 weeks they develop into mature eggs and produce viable offspring. Moreover, we show that simple microinjection of stage I-II follicles with RNA results in reporter gene expression in oocytes and paves the way for developing tools for interfering with maternal gene activity. This early stage oocyte transplantation protocol provides a means to study cellular and molecular aspects of oocyte development in the zebrafish.

Original languageEnglish
Pages (from-to)585-589
Number of pages5
JournalInternational Journal of Developmental Biology
Volume54
Issue number4
DOIs
Publication statusPublished - 2010

Fingerprint

Zebrafish
Oocytes
Embryonic Structures
Transplantation
Mothers
Embryonic Development
Blastula
Genes
Reverse Genetics
Microinjections
Reporter Genes
Eggs
Fishes
Animal Models
RNA
Gene Expression

Keywords

  • Follicle
  • Maternal effect
  • Oogenesis
  • Transgenic
  • Transplantation

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology

Cite this

Csenki, Z., Zaucker, A., Kovács, B., Hadzhiev, Y., Hegyi, Á., Lefler, K. K., ... Müller, F. (2010). Intraovarian transplantation of stage I-II follicles results in viable zebrafish embryos. International Journal of Developmental Biology, 54(4), 585-589. https://doi.org/10.1387/ijdb.082786zc

Intraovarian transplantation of stage I-II follicles results in viable zebrafish embryos. / Csenki, Zsolt; Zaucker, Andreas; Kovács, Balázs; Hadzhiev, Yavor; Hegyi, Árpád; Lefler, Katalin Kinga; Müller, Tamás; Kovács, Róbert; Urbányi, B.; Váradi, László; Müller, Ferenc.

In: International Journal of Developmental Biology, Vol. 54, No. 4, 2010, p. 585-589.

Research output: Contribution to journalArticle

Csenki, Z, Zaucker, A, Kovács, B, Hadzhiev, Y, Hegyi, Á, Lefler, KK, Müller, T, Kovács, R, Urbányi, B, Váradi, L & Müller, F 2010, 'Intraovarian transplantation of stage I-II follicles results in viable zebrafish embryos', International Journal of Developmental Biology, vol. 54, no. 4, pp. 585-589. https://doi.org/10.1387/ijdb.082786zc
Csenki, Zsolt ; Zaucker, Andreas ; Kovács, Balázs ; Hadzhiev, Yavor ; Hegyi, Árpád ; Lefler, Katalin Kinga ; Müller, Tamás ; Kovács, Róbert ; Urbányi, B. ; Váradi, László ; Müller, Ferenc. / Intraovarian transplantation of stage I-II follicles results in viable zebrafish embryos. In: International Journal of Developmental Biology. 2010 ; Vol. 54, No. 4. pp. 585-589.
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