Intracellular targeting of calmodulin mRNAs in primary hippocampal cells

Elod Kortvely, Szilvia Varszegi, Arpad Palfi, K. Gulya

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated the intracellular distribution of the mRNAs corresponding to the three non-allelic CaM genes in cultured hippocampal cells by in situ hybridization with digoxigenin-labeled gene-specific riboprobes. In neurons the perikaryon was heavily stained and strong dendritic mRNA targeting was detected for all three CaM genes. The color labeling exhibited a punctate distribution, suggesting that CaM mRNAs are transported in RNA granules. Immunocytochemistry for S100 demonstrated that glial cells express CaM mRNAs at a very low level. A minority of the cultured cells were negative for either labeling.

Original languageEnglish
Pages (from-to)541-544
Number of pages4
JournalJournal of Histochemistry and Cytochemistry
Volume51
Issue number4
Publication statusPublished - Apr 1 2003

Fingerprint

Calmodulin
Messenger RNA
Cultured Cells
Genes
Digoxigenin
Neuroglia
In Situ Hybridization
Color
Immunohistochemistry
RNA
Neurons

Keywords

  • Calmodulin
  • Dendritic
  • Hippocampal cell culture
  • Intracellular
  • mRNA localization
  • S100 immunocytochemistry

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Intracellular targeting of calmodulin mRNAs in primary hippocampal cells. / Kortvely, Elod; Varszegi, Szilvia; Palfi, Arpad; Gulya, K.

In: Journal of Histochemistry and Cytochemistry, Vol. 51, No. 4, 01.04.2003, p. 541-544.

Research output: Contribution to journalArticle

Kortvely, Elod ; Varszegi, Szilvia ; Palfi, Arpad ; Gulya, K. / Intracellular targeting of calmodulin mRNAs in primary hippocampal cells. In: Journal of Histochemistry and Cytochemistry. 2003 ; Vol. 51, No. 4. pp. 541-544.
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