Intracellular targeting of calmodulin mRNAs in primary hippocampal cells

Elod Kortvely, Szilvia Varszegi, Arpad Palfi, Karoly Gulya

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated the intracellular distribution of the mRNAs corresponding to the three non-allelic CaM genes in cultured hippocampal cells by in situ hybridization with digoxigenin-labeled gene-specific riboprobes. In neurons the perikaryon was heavily stained and strong dendritic mRNA targeting was detected for all three CaM genes. The color labeling exhibited a punctate distribution, suggesting that CaM mRNAs are transported in RNA granules. Immunocytochemistry for S100 demonstrated that glial cells express CaM mRNAs at a very low level. A minority of the cultured cells were negative for either labeling.

Original languageEnglish
Pages (from-to)541-544
Number of pages4
JournalJournal of Histochemistry and Cytochemistry
Volume51
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

    Fingerprint

Keywords

  • Calmodulin
  • Dendritic
  • Hippocampal cell culture
  • Intracellular
  • S100 immunocytochemistry
  • mRNA localization

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this