Intracellular Ca2+ imaging of cultured chicken telencephalic cells: Characterization of ionotropic glutamate receptor activation

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Abstract

In the present study we investigated the intracellular [Ca2+] increasing effect of the excitatory amino acid agonists kainate, AMPA and NMDA on fura-2/AM loaded chicken telencephalic cells in various conditions. Kainate (100 μM) increased [Ca2+]i to 256±23% of the basal level (n=7). In Ca2+-free medium the effect of kainate on intracetlular Ca2+ was completely abolished indicating that the primary source of the Ca2+ signal was the extracellular pool. Voltage dependent Ca2+ channel antagonsim by Cd2+ decreased the intracellular Ca2+ elevation caused by 100 μM kainate indicating the involvement of voltage dependent Ca2+ channels (VDCC).

Original languageEnglish
Pages (from-to)305-307
Number of pages3
JournalActa physiologica Hungarica
Volume84
Issue number3
Publication statusPublished - Dec 1 1996

Keywords

  • Chick neurons
  • Glutamate receptors
  • Intracellular calcium

ASJC Scopus subject areas

  • Physiology (medical)

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