Intracellular calcium redistribution accompanies changes in total tissue Na+, K+ and water during the first two hours of in vitro incubation of hippocampal slices

L. Siklós, U. Kuhnt, A. Párducz, P. Szerdahelyi

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Abstract

Changes of total tissue water, Ca. Na and K contents were monitored in whole transverse hippocampal slices of the guinea-pig during the first 2 h of in vitro incubation. A brief, 75% increase in tissue Ca was noted during the initial 15 min of maintenance, in contrast to a permanent increase of sodium and water contents, coupled to simultaneous decrease of potassium level. The rate of tissue Na, K and water changes comprised a rapid phase at the first 10-20 min, parallel with the increase of the tissue Ca content, and a slow phase during the rest of the incubation period. Development of specific morphological alterations, representative of ischemic/hypoxic lesions and a translocation of calcium from cytoplasm to mitochondria and endoplasmic reticulum during slice maintenance, was also detected by electron microscopy. A two-step mechanism might explain the development of a new steady-state total calcium content of slices, in which the cellular Ca2+ uptake at the beginning of incubation, likely triggered by hypoxic/ischemic trauma of slice preparation, is followed by a balanced Ca2+ influx, extrusion and sequestration (predominantly into mitochondria and endoplasmic reticulum) during maintenance.

Original languageEnglish
Pages (from-to)1013-1022
Number of pages10
JournalNeuroscience
Volume79
Issue number4
DOIs
Publication statusPublished - Jun 6 1997

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Calcium
Water
Maintenance
Endoplasmic Reticulum
Mitochondria
Electron Microscopy
Potassium
Guinea Pigs
Cytoplasm
Sodium
In Vitro Techniques
Wounds and Injuries

Keywords

  • Calcium
  • Hippocampal slice
  • Incubation
  • Ionic homeostasis
  • Ischemia
  • Ultrastructure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "Changes of total tissue water, Ca. Na and K contents were monitored in whole transverse hippocampal slices of the guinea-pig during the first 2 h of in vitro incubation. A brief, 75{\%} increase in tissue Ca was noted during the initial 15 min of maintenance, in contrast to a permanent increase of sodium and water contents, coupled to simultaneous decrease of potassium level. The rate of tissue Na, K and water changes comprised a rapid phase at the first 10-20 min, parallel with the increase of the tissue Ca content, and a slow phase during the rest of the incubation period. Development of specific morphological alterations, representative of ischemic/hypoxic lesions and a translocation of calcium from cytoplasm to mitochondria and endoplasmic reticulum during slice maintenance, was also detected by electron microscopy. A two-step mechanism might explain the development of a new steady-state total calcium content of slices, in which the cellular Ca2+ uptake at the beginning of incubation, likely triggered by hypoxic/ischemic trauma of slice preparation, is followed by a balanced Ca2+ influx, extrusion and sequestration (predominantly into mitochondria and endoplasmic reticulum) during maintenance.",
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AU - Siklós, L.

AU - Kuhnt, U.

AU - Párducz, A.

AU - Szerdahelyi, P.

PY - 1997/6/6

Y1 - 1997/6/6

N2 - Changes of total tissue water, Ca. Na and K contents were monitored in whole transverse hippocampal slices of the guinea-pig during the first 2 h of in vitro incubation. A brief, 75% increase in tissue Ca was noted during the initial 15 min of maintenance, in contrast to a permanent increase of sodium and water contents, coupled to simultaneous decrease of potassium level. The rate of tissue Na, K and water changes comprised a rapid phase at the first 10-20 min, parallel with the increase of the tissue Ca content, and a slow phase during the rest of the incubation period. Development of specific morphological alterations, representative of ischemic/hypoxic lesions and a translocation of calcium from cytoplasm to mitochondria and endoplasmic reticulum during slice maintenance, was also detected by electron microscopy. A two-step mechanism might explain the development of a new steady-state total calcium content of slices, in which the cellular Ca2+ uptake at the beginning of incubation, likely triggered by hypoxic/ischemic trauma of slice preparation, is followed by a balanced Ca2+ influx, extrusion and sequestration (predominantly into mitochondria and endoplasmic reticulum) during maintenance.

AB - Changes of total tissue water, Ca. Na and K contents were monitored in whole transverse hippocampal slices of the guinea-pig during the first 2 h of in vitro incubation. A brief, 75% increase in tissue Ca was noted during the initial 15 min of maintenance, in contrast to a permanent increase of sodium and water contents, coupled to simultaneous decrease of potassium level. The rate of tissue Na, K and water changes comprised a rapid phase at the first 10-20 min, parallel with the increase of the tissue Ca content, and a slow phase during the rest of the incubation period. Development of specific morphological alterations, representative of ischemic/hypoxic lesions and a translocation of calcium from cytoplasm to mitochondria and endoplasmic reticulum during slice maintenance, was also detected by electron microscopy. A two-step mechanism might explain the development of a new steady-state total calcium content of slices, in which the cellular Ca2+ uptake at the beginning of incubation, likely triggered by hypoxic/ischemic trauma of slice preparation, is followed by a balanced Ca2+ influx, extrusion and sequestration (predominantly into mitochondria and endoplasmic reticulum) during maintenance.

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