Intracellular calcium and outer hair cell electromotility

Magdolna Szönyi, David Z Z He, Ottó Ribári, I. Sziklai, Peter Dallos

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The influence of increased intracellular calcium level on outer hair cell (OHC) electromotility was examined by means of transcellular electrical stimulation in a partitioning microchamber. Electromotile activity was measured before and after application of the calcium ionophore ionomycin, which promotes the inflow of extracellular calcium, as well as its release from intracellular calcium stores. The ionomycin solvent, dimethyl sulphoxide (DMSO), by itself elicited a significant decrease in the magnitude of OHC electromotility. The DMSO effect was counteracted by 10 μM ionomycin and was reversed by 50 μM ionomycin. The increase in electromotility is partially mediated by a calmodulin-dependent mechanism, since W7, a calmodulin antagonist, attenuated the 50 μM ionomycin-induced motility increase. Our results suggest that the electromotility magnitude increase in isolated OHCs due to ionomycin is a calcium/calmodulin-dependent phenomenon.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalBrain Research
Volume922
Issue number1
DOIs
Publication statusPublished - Dec 13 2001

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Outer Auditory Hair Cells
Ionomycin
Calcium
Calmodulin
Dimethyl Sulfoxide
Calcium Ionophores
Electric Stimulation

Keywords

  • Calcium
  • Electromotility
  • Ionomycin
  • Outer hair cell
  • Signal transduction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Intracellular calcium and outer hair cell electromotility. / Szönyi, Magdolna; He, David Z Z; Ribári, Ottó; Sziklai, I.; Dallos, Peter.

In: Brain Research, Vol. 922, No. 1, 13.12.2001, p. 65-70.

Research output: Contribution to journalArticle

Szönyi, Magdolna ; He, David Z Z ; Ribári, Ottó ; Sziklai, I. ; Dallos, Peter. / Intracellular calcium and outer hair cell electromotility. In: Brain Research. 2001 ; Vol. 922, No. 1. pp. 65-70.
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