Intracarotid tumor necrosis factor-α administration increases the blood-brain barrier permeability in cerebral cortex of the newborn pig

Quantitative aspects of double-labelling studies and confocal laser scanning analysis

C. Ábrahám, M. Deli, Ferenc Joó, Pál Megyeri, Gérard Torpier

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Abstract

Tumor necrosis factor-α (TNF-α) plays a crucial role in the pathogenesis of the central nervous system infections. The aim of the present study was to analyze quantitatively the changes in the blood-brain barrier (BBB) permeability after the intracarotid injection of TNF-α. Recombinant human TNF-α was injected into the left internal carotid artery of anesthetized newborn pigs (n = 48) in the doses of 0, 1000, 10 000 and 100 000 IU, respectively. Before, as well as 1, 2, 4, 8, and 16 h after the challenge, the extravasation of a small (sodium fluorescein (SF), mw 376), and a large (Evan's blue-albumin (EBA), mw 67 000) tracer was determined concomitantly by spectrophotometry in the cerebral cortex of the animals. There was a time- and dose-dependent increase in BBB permeability both for SF and EBA; however, significant (P <0.05) BBB opening for albumin only developed 2 h after the challenge. In the morphological study the same excitable tracers, identical experimental protocol and groups were used. Cryostat sections of brain tissue were viewed for optical sectioning with a confocal laser scanning microscope equipped with an argon/krypton ion laser. A diffuse BBB opening for SF and a moderate perivascular extravasation for EBA were found in the cortices of TNF-α-treated animals. We conclude that significant increases in intravascular TNF-α-concentration during neonatal infections may result in vasogenic brain edema formation.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalNeuroscience Letters
Volume208
Issue number2
DOIs
Publication statusPublished - Apr 19 1996

Fingerprint

Blood-Brain Barrier
Cerebral Cortex
Evans Blue
Albumins
Permeability
Lasers
Swine
Tumor Necrosis Factor-alpha
Fluorescein
Krypton
Central Nervous System Infections
Gas Lasers
Spectrophotometry
Brain Edema
Internal Carotid Artery
Injections
Brain
Infection

Keywords

  • Blood-brain barrier
  • Brain edema
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Intracarotid tumor necrosis factor-α administration increases the blood-brain barrier permeability in cerebral cortex of the newborn pig: Quantitative aspects of double-labelling studies and confocal laser scanning analysis",
abstract = "Tumor necrosis factor-α (TNF-α) plays a crucial role in the pathogenesis of the central nervous system infections. The aim of the present study was to analyze quantitatively the changes in the blood-brain barrier (BBB) permeability after the intracarotid injection of TNF-α. Recombinant human TNF-α was injected into the left internal carotid artery of anesthetized newborn pigs (n = 48) in the doses of 0, 1000, 10 000 and 100 000 IU, respectively. Before, as well as 1, 2, 4, 8, and 16 h after the challenge, the extravasation of a small (sodium fluorescein (SF), mw 376), and a large (Evan's blue-albumin (EBA), mw 67 000) tracer was determined concomitantly by spectrophotometry in the cerebral cortex of the animals. There was a time- and dose-dependent increase in BBB permeability both for SF and EBA; however, significant (P <0.05) BBB opening for albumin only developed 2 h after the challenge. In the morphological study the same excitable tracers, identical experimental protocol and groups were used. Cryostat sections of brain tissue were viewed for optical sectioning with a confocal laser scanning microscope equipped with an argon/krypton ion laser. A diffuse BBB opening for SF and a moderate perivascular extravasation for EBA were found in the cortices of TNF-α-treated animals. We conclude that significant increases in intravascular TNF-α-concentration during neonatal infections may result in vasogenic brain edema formation.",
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T2 - Quantitative aspects of double-labelling studies and confocal laser scanning analysis

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AU - Deli, M.

AU - Joó, Ferenc

AU - Megyeri, Pál

AU - Torpier, Gérard

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